Ben Rhouma Khémais, Marrakchi Hassen, Khouja Habib, Attalah Khaled, Ben Miled Elyas, Sakly Mohsen
Laboratory of Physiology of Reproduction, Science Faculty of Bizerte, 7021 Jarzouna, Bizerte, Tunisia.
J Reprod Med. 2003 May;48(5):349-54.
To compare the outcomes of intracytoplasmic sperm injection (ICSI) with fresh and cryopreserved testicular spermatozoa in patients with obstructive and nonobstructive azoospermia.
Retrospective analysis.
A total of 60 ICSI treatment cycles (32 fresh, 28 frozen) using testicular sperm obtained by open biopsy were analyzed retrospectively. No statistically significant differences were noted in any of the parameters examined between ICSI cycles from fresh and frozen-thawed testicular spermatozoa. Fertilization rates were 61.9% with fresh vs. 61.7% with frozen-thawed testicular sperm, pregnancy rates per embryo transfer 29.1% vs. 25.0%, respectively, and delivery rates 85.7% vs. 80.0%, respectively.
Intracytoplasmic sperm injection with frozen-thawed testicular spermatozoa permits the obtaining of satisfactory fertilization and pregnancy rates, and the cryopreserved sperm can be used as an effective sperm source in future ICSI cycles.
比较梗阻性和非梗阻性无精子症患者采用新鲜和冷冻保存的睾丸精子进行卵胞浆内单精子注射（ICSI）的结果。
回顾性分析。
对通过开放活检获取的睾丸精子进行的60个ICSI治疗周期（32个新鲜周期，28个冷冻周期）进行回顾性分析。新鲜和冻融睾丸精子的ICSI周期之间，在所检查的任何参数上均未发现统计学上的显著差异。新鲜睾丸精子的受精率为61.9%，冻融睾丸精子的受精率为61.7%；每次胚胎移植的妊娠率分别为29.1%和25.0%；分娩率分别为85.7%和80.0%。
采用冻融睾丸精子进行卵胞浆内单精子注射可获得满意的受精率和妊娠率，冷冻保存的精子可作为未来ICSI周期的有效精子来源。
