Ito Kazuaki, Takayama Yohichi, Makabe Nobuyuki, Mitsui Ryo, Hirokawa Takeshi
Department of Biotechnology and Chemistry, School of Engineering, Kinki University, 1 Umenobe, Takaya, Higashi-Hiroshima 739-2116, Japan.
J Chromatogr A. 2005 Aug 12;1083(1-2):63-7. doi: 10.1016/j.chroma.2005.05.073.
A fast and highly sensitive ion chromatographic method using monolithic ODS columns was developed for the determination of nitrite (NO2-) and nitrate (NO3-) in seawater. Two monolithic ODS columns (50 mm x 4.6 mm i.d. + 100 mm x 4.6 mm i.d.) connected in series were coated and equilibrated with 5 mM cetyltrimethylammonium chloride (CTAC) aqueous solution. The column efficiency with 0.5 M NaCl as the mobile phase did not decrease in spite of the increase in flow rate of the mobile phase. Thus, good chromatograms were obtained within 3 minutes for NO2- and NO3 in artificial seawater without interferences by coexisting ions. The detection limit (S/N = 3) with UV detection at 225 nm was 0.8 and 1.6 microg/L for NO2- and NO3-, respectively. The characteristics of the monolithic CTA(+)-coated ODS columns were discussed. The present method was successfully applied to the fast and sensitive determination of NO2- and NO3- in real seawater samples.
建立了一种使用整体式ODS柱的快速、高灵敏度离子色谱法，用于测定海水中的亚硝酸盐（NO2-）和硝酸盐（NO3-）。将两根串联的整体式ODS柱（50 mm×4.6 mm内径 + 100 mm×4.6 mm内径）用5 mM十六烷基三甲基氯化铵（CTAC）水溶液进行涂覆和平衡。尽管流动相流速增加，但以0.5 M NaCl为流动相时柱效并未降低。因此，人工海水中的NO2-和NO3-在3分钟内即可获得良好的色谱图，且不受共存离子的干扰。在225 nm处进行紫外检测时，NO2-和NO3-的检测限（S/N = 3）分别为0.8和1.6 μg/L。讨论了整体式CTA(+)-涂覆ODS柱的特性。该方法成功应用于实际海水样品中NO2-和NO3-的快速、灵敏测定。
