Trösken Eva R, Bittner Nataly, Völkel Wolfgang
Department of Toxicology, University of Würzburg, Versbacher Str. 9, 97078 Würzburg, Germany.
J Chromatogr A. 2005 Aug 12;1083(1-2):113-9. doi: 10.1016/j.chroma.2005.06.020.
A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method has been developed for the direct quantitation of residues of 13 azole fungicides in wine and has been successfully applied to 103 wine samples. The method utilizing 25 transitions is highly sensitive and specific with centrifugation as the only sample work-up step. Precision was better than 14% and accuracy ranged between 80 and 120%. Quantitation limits (LOQs) ranged between 0.25 (penconazole) and 7.5 ng/mL (triadimefon). Since the LOQs achieved are at least four times lower than the maximum residue levels for azole-fungicides in wine prescribed, the method presented here can be conveniently used as a screening assay for azole-residues in wine samples.
已开发出一种液相色谱-串联质谱（LC-MS/MS）方法，用于直接定量葡萄酒中13种唑类杀菌剂的残留量，并已成功应用于103个葡萄酒样品。该方法利用25种跃迁，以离心作为唯一的样品预处理步骤，具有高灵敏度和特异性。精密度优于14%，准确度在80%至120%之间。定量限（LOQ）在0.25（戊唑醇）至7.5 ng/mL（三唑酮）之间。由于所达到的定量限至少比规定的葡萄酒中唑类杀菌剂的最大残留量低四倍，因此本文提出的方法可方便地用作葡萄酒样品中唑类残留的筛选分析方法。
