Zhang Hai-Ying, Liu Yong, Liu Dong-Cheng, Wang Xiu-Zhi, Wang Chao, Wang Ling-Xia, Zhang Ai-Min, Li Ping
Institute of Genetics and Developmental Biology, Chinese Academy of Science, Beijing 100101, China.
Yi Chuan Xue Bao. 2005 Jul;32(7):719-25.
Rice blast caused by Magnaporthe grisea is one of the most serious constraints on high productivity. Understanding the mechanism of the infection of Magnaporthe grisea and the change of gene expression after infection is useful to control blast disease in rice. This work presents the isolation of differentially expressed cDNA fragments from rice leaf induced by the inoculum suspension of Magnaporthe grisea using mRNA differential display technique. Total 87 differential expressed cDNA fragments were recoveried and reamplified. The dot-blotting results showed that 6 fragments of 81 were confirmed to be the expression induced by Magnaporthe grisea inoculum. Those fragments were then cloned into vectors for sequencing. Sequence analysis through Internet Blast searching showed that 3 fragments were novel gene fragments. One was homologous with a putative malate synthase gene on rice chromosome 4 with 78% identities of amino acid; one was highly homologous (75% identity) with rice RPR1 gene on chromosome 11, which has a conservative structure of NBS-LRR domain and may be related to signal transduction of rice defense reaction;another one was homologous with a putative thioredoxin gene on rice chromosome 6 with the identity of 72%.
由稻瘟病菌引起的稻瘟病是限制水稻高产的最严重因素之一。了解稻瘟病菌的侵染机制以及侵染后基因表达的变化，对于控制水稻稻瘟病具有重要意义。本研究利用mRNA差异显示技术，从稻瘟病菌接种悬浮液诱导的水稻叶片中分离差异表达的cDNA片段。共回收并重新扩增了87个差异表达的cDNA片段。斑点杂交结果显示，81个片段中有6个被确认为稻瘟病菌接种诱导表达的片段。随后将这些片段克隆到载体中进行测序。通过互联网Blast搜索进行的序列分析表明，有3个片段是新的基因片段。一个与水稻第4号染色体上的一个假定苹果酸合酶基因同源，氨基酸序列一致性为78%；一个与水稻第11号染色体上的RPR1基因高度同源（一致性为75%），该基因具有保守的NBS-LRR结构域，可能与水稻防御反应的信号转导有关；另一个与水稻第6号染色体上的一个假定硫氧还蛋白基因同源，一致性为72%。
