Liu Zhao-Hua, Zhang Zhen-Shan, Guo Hong-Nian, Jia Yan-Tao, Zheng Guang-Yu, Tian Ying-Chuan
Institution of Microbiology, Chinese Academy of Sciences, National Key Laboratory of Plant Genomics, Beijing 100080, China.
Yi Chuan Xue Bao. 2005 Jul;32(7):758-63.
A plant expression vector pBACG containing the DNA sequence coding for Amaranthus caudatus agglutinin (ACA) and a modified Glanthus nivalis agglutinin (GNA) gene was constructed. Leaf explants of Nicotiana tobacum cv. SRI were transformed with A. tumefaciens LBA4404 harbouring the above expression vector. Results from PCR and Southern blotting analysis showed that both the ACA and GNA gene were inserted into the genome of transformed tobacco plants. Western blottingting analysis of soluble protein isolated from transgenic plants showed that ACA and GNA were synthesized. The results from insect bioassay with peach aphids ( Myzus persicae) revealed that the transgenic plants of pBACG had acquired high resistance against peach aphids. The average aphid-inhibition rate reached up to 83.9% and 85.3% for transgenic plants (T0) and their selfed progenies (T1) respectively,indicating that the functions of these two genes were inheritable.
构建了一种植物表达载体pBACG，其包含编码尾穗苋凝集素（ACA）的DNA序列和一个修饰的雪花莲凝集素（GNA）基因。用携带上述表达载体的根癌农杆菌LBA4404转化烟草品种Nicotiana tobacum cv. SRI的叶片外植体。PCR和Southern杂交分析结果表明，ACA和GNA基因均已插入到转化烟草植株的基因组中。对从转基因植株中分离的可溶性蛋白进行的Western杂交分析表明，ACA和GNA已合成。用桃蚜（Myzus persicae）进行的昆虫生物测定结果显示，pBACG转基因植株对桃蚜具有高抗性。转基因植株（T0）及其自交后代（T1）的平均蚜虫抑制率分别高达83.9%和85.3%，表明这两个基因的功能是可遗传的。
