Mino Yoshiki, Yukita Mayumi, Hiratsuka Nobuhiro, Wariishi Hiroyuki
Department of Environment Analysis, Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka 569-1094, Japan.
Biol Pharm Bull. 2005 Aug;28(8):1535-8. doi: 10.1248/bpb.28.1535.
The complete amino acid sequences of [2Fe-2S] ferredoxin from Atropa belladonna and Hyoscyamus niger have been determined by automated Edman degradation of the entire S-carboxymethylcysteinyl proteins and of the peptides obtained by enzymatic digestion. These two ferredoxins exhibited 1-8 differences in their amino acid sequences compared to those of other tropane-alkaloid-containing plants (Scopolia japonica, Datura stramonium, D. metel, and D. arborea), and only 1 or 4 differences compared to S. japonica and D. arborea. In contrast, 9-23 differences were observed among the other solanaceous ferredoxins. This suggests that tropane-alkaloid-containing plants are closely related taxonomically.
通过对完整的S-羧甲基半胱氨酰化蛋白质及其酶切所得肽段进行自动Edman降解，已确定了颠茄和黑莨菪中[2Fe-2S]铁氧化还原蛋白的完整氨基酸序列。与其他含托烷生物碱的植物（日本东莨菪、曼陀罗、木本曼陀罗和毛曼陀罗）相比，这两种铁氧化还原蛋白的氨基酸序列存在1-8个差异，与日本东莨菪和毛曼陀罗相比仅存在1个或4个差异。相比之下，在其他茄科铁氧化还原蛋白中观察到9-23个差异。这表明含托烷生物碱的植物在分类学上密切相关。
