Lee Byeong Chun, Kim Min Kyu, Jang Goo, Oh Hyun Ju, Yuda Fibrianto, Kim Hye Jin, Hossein M Shamim, Kim Jung Ju, Kang Sung Keun, Schatten Gerald, Hwang Woo Suk
Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Gwanak-gu, Seoul 151-742, South Korea.
Nature. 2005 Aug 4;436(7051):641. doi: 10.1038/436641a.
Several mammals--including sheep, mice, cows, goats, pigs, rabbits, cats, a mule, a horse and a litter of three rats--have been cloned by transfer of a nucleus from a somatic cell into an egg cell (oocyte) that has had its nucleus removed. This technology has not so far been successful in dogs because of the difficulty of maturing canine oocytes in vitro. Here we describe the cloning of two Afghan hounds by nuclear transfer from adult skin cells into oocytes that had matured in vivo. Together with detailed sequence information generated by the canine-genome project, the ability to clone dogs by somatic-cell nuclear transfer should help to determine genetic and environmental contributions to the diverse biological and behavioural traits associated with the many different canine breeds.
包括绵羊、小鼠、奶牛、山羊、猪、兔子、猫、一匹骡子、一匹马以及一窝三只大鼠在内的几种哺乳动物，已通过将体细胞的细胞核转移到已去除细胞核的卵细胞（卵母细胞）中而被克隆。由于犬类卵母细胞在体外成熟存在困难，这项技术目前在狗身上尚未成功。在此，我们描述了通过将成年皮肤细胞的细胞核转移到在体内成熟的卵母细胞中，克隆出两只阿富汗猎犬的过程。结合犬类基因组计划生成的详细序列信息，通过体细胞核移植克隆狗的能力应有助于确定基因和环境对与众多不同犬种相关的各种生物学和行为特征的影响。
