Rapid penetration of lucifer yellow into vital teeth and dye coupling between odontoblasts and neighbouring pulp cells in the cat.

The location and long process of odontoblasts appear well suited to detection of external stimuli. The odontoblasts may transmit the information not individually but as a syncytium via gap junction, which functions as a mechanism for intercellular linking cells and as the route for dye coupling. The aims of the present study were to examine dye penetration through enamel and dentin, and to confirm dye coupling between odontoblasts (OBs) or between odontoblasts and other pulpal cells beneath the odontoblastic layer (PCs). Either lucifer yellow (LY) or borate buffer (control) was applied to etched enamel surface of feline canines for 30 min at atmospheric pressure. In the decalcified sections, lucifer yellow positive cells were found not only in but also beneath the odontoblastic layer (experiment 1). In the isolated pulp cells, all OBs (27/27) and some PCs (6/9) that were immunocytochemically differentiated using two monoclonal antibodies were labelled with LY (experiment 2). These results indicate the remarkably quick movement of LYE through enamel and dentin into the superficial pulp. In experiment 3, fresh OBs and PCs were isolated from feline canines to which LY had not been applied. LY was iontophoretically injected into an OB-like cell that had an oval cell body and a long monopolar process. Some PCs and OBs identified immunocytochemically were labelled with LY, with the exception of a few LY-negative cells. These findings indicate that dye coupling exists not only between OBs but also between OBs and PCs. Thus, the coupling provides evidence for a functional link via which information is transmitted between OBs and PCs.