Ciray H N, Persson B E, Roomans G M, Ulmsten U
Department of Human Anatomy, University of Uppsala, Sweden.
Cell Biol Int. 1995 Jul;19(7):609-17. doi: 10.1006/cbir.1995.1108.
Term pregnant human myometrial cells in whole mounts were microinjected by pressure with the fluorescent probes Lucifer Yellow and carboxyfluorescein. Tissues obtained from acute and elective sections displayed weak dye-coupling when injected with Lucifer Yellow. Injection of carboxyfluorescein into cells from the elective sections resulted in a more extensive dye-coupling than that observed with Lucifer Yellow. These results indicate that term pregnant human myometrial cells are metabolically coupled before labor and carboxyfluorescein is superior to Lucifer Yellow in detecting the coupling.
采用压力微注射法,将荧光探针路西法黄和羧基荧光素注入足月妊娠人类子宫肌层全层细胞。急性切片和择期切片获取的组织在注入路西法黄后显示出较弱的染料偶联。将羧基荧光素注入择期切片的细胞后,染料偶联比注入路西法黄时更为广泛。这些结果表明,足月妊娠人类子宫肌层细胞在分娩前存在代谢偶联,且在检测偶联方面,羧基荧光素优于路西法黄。