白血病抑制因子信号传导与下丘脑-垂体-肾上腺轴的胚胎发育有关。

Leukemia inhibitory factor signaling is implicated in embrionic development of the HPA axis.

作者信息

Ware Carol B, Kariagina Anastasia, Zonis Svetlana, Alon Dana, Chesnokova Vera

机构信息

Department of Comparative Medicine, University of Washington, Seattle, WA 98195-7190, USA.

出版信息

FEBS Lett. 2005 Aug 15;579(20):4465-9. doi: 10.1016/j.febslet.2005.07.014.

DOI:10.1016/j.febslet.2005.07.014

PMID:16081071

Abstract

Leukemia inhibitory factor (LIF) is expressed in the hypothalamic-pituitary adrenal (HPA) axis and stimulates pituitary POMC transcription. The role of LIF receptor signaling in HPA axis development was examined. Lifr -/- and Lifr +/+ fetuses were obtained by Cesarean section on E18.5. Despite a 3-fold induction of hypothalamic CRH mRNA, pituitary POMC mRNA and RU486-induced ACTH levels were decreased in Lifr -/- mice. High CRH may be caused by increased central TNFalpha and IL-6 expression. Lifr -/- mice demonstrate elevated pituitary glucocorticoid (GR) and mineralocorticoid (MR) receptor mRNA and protein levels, indicating the importance of LIF signaling for HPA axis development.

摘要

白血病抑制因子（LIF）在下丘脑-垂体-肾上腺（HPA）轴中表达，并刺激垂体促肾上腺皮质激素原（POMC）转录。研究了LIF受体信号在HPA轴发育中的作用。在胚胎第18.5天通过剖宫产获得Lifr -/- 和Lifr +/+ 胎儿。尽管Lifr -/- 小鼠下丘脑促肾上腺皮质激素释放激素（CRH）mRNA诱导增加了3倍，但垂体POMC mRNA和RU486诱导的促肾上腺皮质激素（ACTH）水平却降低了。高CRH可能是由中枢肿瘤坏死因子α（TNFα）和白细胞介素6（IL-6）表达增加引起的。Lifr -/- 小鼠垂体糖皮质激素（GR）和盐皮质激素（MR）受体mRNA及蛋白水平升高，表明LIF信号对HPA轴发育很重要。