Coudron Philip E
Pathology and Laboratory Medicine Service/113, McGuire Veterans Affairs Medical Center, Richmond, VA 23249-0001, USA.
J Clin Microbiol. 2005 Aug;43(8):4163-7. doi: 10.1128/JCM.43.8.4163-4167.2005.
Non-beta-lactam inhibitor-based methods were evaluated for detecting plasmid-mediated AmpC beta-lactamases in Klebsiella spp., Escherichia coli, and Proteus mirabilis. Using CLSI methodology and disks containing cefotetan alone and in combination with 400 mug of boronic acid, 9 of 10 positive control strains and 54 of 55 AmpC-PCR-positive clinical isolates were detected. Importantly 71% and 40% of these clinical isolates were susceptible by routine testing to ceftriaxone and ceftazidime, respectively. Boronic acid disks also enhanced detection of expanded-spectrum beta-lactamases in AmpC producers.
对基于非β-内酰胺酶抑制剂的方法进行了评估，以检测肺炎克雷伯菌、大肠埃希菌和奇异变形杆菌中质粒介导的AmpCβ-内酰胺酶。采用临床和实验室标准协会（CLSI）方法，使用单独含头孢替坦以及与400μg硼酸联用的纸片，检测出10株阳性对照菌株中的9株以及55株AmpC-PCR阳性临床分离株中的54株。重要的是，这些临床分离株中分别有71%和40%通过常规检测对头孢曲松和头孢他啶敏感。硼酸纸片还增强了对产AmpC酶菌株中广谱β-内酰胺酶的检测。
