Cai Zhao, Manavis Jim, Cash Kathy, Thompson Philip D, Blumbergs Peter C
Centre for Neurological Disease, Hanson Institute, Adelaide, Australia.
Appl Immunohistochem Mol Morphol. 2005 Sep;13(3):292-4. doi: 10.1097/01.pai.0000140016.83608.ed.
The authors describe a simple and cost-effective method of immunostaining semithin epoxy resin sections of peripheral nerve for light microscopy with antibodies to myelin protein zero, peripheral myelin protein 22, myelin basic protein, and neurofilament protein 200 using a combined technique of surface etching with sodium ethoxide and heat-induced antigen retrieval.
作者描述了一种简单且经济高效的免疫染色方法，该方法利用乙醇钠表面蚀刻和热诱导抗原修复的联合技术，使用针对髓磷脂蛋白零、外周髓磷脂蛋白22、髓磷脂碱性蛋白和神经丝蛋白200的抗体，对周围神经的半薄环氧树脂切片进行光镜检查。
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