使用基于寡核苷酸阵列的转录因子分析（OATFA）对活性转录因子进行平行分析。

Parallel profiling of active transcription factors using an oligonucleotide array-based transcription factor assay (OATFA).

作者信息

Shao Wei, Wei Hua-Jiang, Qiao Ji-Ying, Zhao Yong-Chao, Sun Yi-Min, Zhou Yu-Xiang, Cheng Jing

机构信息

Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China.

出版信息

J Proteome Res. 2005 Jul-Aug;4(4):1451-6. doi: 10.1021/pr050053l.

DOI:10.1021/pr050053l

PMID:16083299

Abstract

Cell/tissue-specific gene expression are tightly regulated by various combinations of multiple transcription factors (TFs). Here, we present an oligonucleotide array-based transcription factor assay (OATFA), which allows the simultaneous assay of multiple active TFs. In this proof-of-principle work, both purified TFs and cell extracts were analyzed using OATFA and further antibody-based validation confirmed the chip data. This method could simplify the assay of multiple TFs and may facilitate high-throughput profiling of large numbers of TFs.

摘要

细胞/组织特异性基因表达受到多种转录因子（TFs）不同组合的严格调控。在此，我们展示了一种基于寡核苷酸阵列的转录因子检测方法（OATFA），该方法能够同时检测多种活性转录因子。在这项原理验证工作中，使用OATFA对纯化的转录因子和细胞提取物进行了分析，进一步基于抗体的验证证实了芯片数据。该方法可以简化多种转录因子的检测，并可能有助于对大量转录因子进行高通量分析。