Möller André, Avila Frank W, Erickson James W, Jäckle Herbert
Abteilung Molekulare Entwicklungsbiologie, Max-Planck-Institut für biophysikalische Chemie, Am Fassberg, D-37077 Göttingen, Germany.
J Mol Biol. 2005 Sep 16;352(2):329-37. doi: 10.1016/j.jmb.2005.07.009.
The SWI/SNF-like chromatin remodeling complex of Drosophila, the Brahma complex, contains four subunits (Brahma, BAP155/Moira, SNR1 and BAP60) conserved from yeast to humans. A reconstituted human complex lacking the BAP60 homolog shows full remodeling activity, suggesting that BAP60 is not essential for the core function. We generated Drosophila mutants and found that BAP60 carries a vital function and participates in complex-mediated transcriptional activation and repression. BAP60 binds DNA and shows genetic and physical interactions with the sex-determining transcription factors encoded by sisterless A and scute. The results support the conclusion that BAP60 participates in site-specific recruitment of the Brahma complex in Drosophila.
果蝇的SWI/SNF样染色质重塑复合体，即Brahma复合体，包含四个从酵母到人类都保守的亚基（Brahma、BAP155/莫伊拉、SNR1和BAP60）。一种缺乏BAP60同源物的重组人类复合体显示出完全的重塑活性，这表明BAP60对于核心功能并非必不可少。我们生成了果蝇突变体，发现BAP60具有重要功能，并参与复合体介导的转录激活和抑制。BAP60结合DNA，并与无姐妹A和scute编码的性别决定转录因子表现出遗传和物理相互作用。这些结果支持了BAP60参与果蝇中Brahma复合体的位点特异性募集这一结论。
