2002年海豹瘟热疫情期间从德国北海港湾海豹（Phoca vitulina）分离出的马链球菌兽疫亚种的表型和基因型特性

Phenotypic and genotypic properties of Streptococcus equi subsp. zooepidemicus isolated from harbor seals (Phoca vitulina) from the German North Sea during the phocine distemper outbreak in 2002.

作者信息

Akineden O, Hassan A A, Alber J, El-Sayed A, Estoepangestie A T S, Lämmler C, Weiss R, Siebert U

机构信息

Institut für Tierärztliche Nahrungsmittelkunde, Justus-Liebig-Universität Giessen, Giessen, Germany.

出版信息

Vet Microbiol. 2005 Sep 30;110(1-2):147-52. doi: 10.1016/j.vetmic.2005.06.010.

DOI:10.1016/j.vetmic.2005.06.010

PMID:16084036

Abstract

The present study was designed to identify and compare 32 beta-hemolytic streptococci isolated from 28 different harbor seals of the German North Sea during the phocine distemper outbreak in 2002. The bacteria were identified as Streptococcus equi subsp. zooepidemicus based on cultural, biochemical, serological and molecular studies. Epidemiological investigations by PCR restriction fragment length polymorphism analysis of the 16S-23S rDNA intergenic spacer region and gene szp and by macrorestriction analysis of the chromosomal DNA of the strains by pulsed field gel electrophoresis revealed that all 32 strains appeared to be identical. These results indicate that a single bacterial clone seemed to be distributed among the harbor seal population of the German North Sea during this outbreak.

摘要

本研究旨在鉴定和比较2002年海豹瘟热疫情期间从德国北海28只不同斑海豹中分离出的32株β-溶血性链球菌。通过培养、生化、血清学和分子研究，这些细菌被鉴定为马链球菌兽疫亚种。通过对16S-23S rDNA基因间隔区和szp基因进行PCR限制性片段长度多态性分析，以及通过脉冲场凝胶电泳对菌株染色体DNA进行宏观限制性分析进行流行病学调查，结果显示所有32株菌株似乎是相同的。这些结果表明，在此次疫情期间，一个单一的细菌克隆似乎在德国北海的斑海豹种群中传播。