用简单荧光试验检测布氏锥虫对含砷药物的耐药性。
Detection of arsenical drug resistance in Trypanosoma brucei with a simple fluorescence test.
作者信息
Stewart Mhairi L, Krishna Sanjeev, Burchmore Richard J S, Brun Reto, de Koning Harry P, Boykin David W, Tidwell Richard R, Hall J Ed, Barrett Michael P
机构信息
University of Glasgow, Division of Infection and Immunity, Institute of Biomedical and Life Sciences, The Joseph Black Building, Glasgow G12 8QQ, UK.
出版信息
Lancet. 2005;366(9484):486-7. doi: 10.1016/S0140-6736(05)66793-1.
The resurgence of human African trypanosomiasis (HAT), coupled with an increased incidence of drug resistance, is of concern. We report a quick, simple, and sensitive test for identification of parasites resistant to melarsoprol, the main drug used to treat late stage HAT. Resistant parasites are defective in a plasma membrane transporter responsible for drug uptake. The same transporter carries the fluorescent diamidine DB99 (2,5-bis-(4-amidinophenyl)-3,4-dimethylfuran) into trypanosomes. The two DNA-containing structures in the trypanosome--the nucleus and the kinetoplast--begin to fluoresce within 1 min of introduction of DB99, unless drug resistant.
人类非洲锥虫病（HAT）的死灰复燃，再加上耐药性发病率的上升，令人担忧。我们报告了一种快速、简单且灵敏的检测方法，用于鉴定对美拉胂醇耐药的寄生虫，美拉胂醇是治疗晚期HAT的主要药物。耐药寄生虫在负责药物摄取的质膜转运蛋白上存在缺陷。同一种转运蛋白将荧光双脒DB99（2,5-双-（4-脒基苯基）-3,4-二甲基呋喃）转运到锥虫体内。锥虫体内的两个含DNA结构——细胞核和动基体——在引入DB99后1分钟内开始发出荧光，除非是耐药的。
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