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稻壳滤液作为黑脱硫肠状菌生长的营养培养基:特性及硫酸盐还原研究

Rice husk filtrate as a nutrient medium for the growth of Desulfotomaculum nigrificans: characterisation and sulfate reduction studies.

作者信息

Chockalingam Evvie, Sivapriya K, Subramanian S, Chandrasekaran S

机构信息

Department of Metallurgy, Indian Institute of Science, Bangalore 560 012, India.

出版信息

Bioresour Technol. 2005 Nov;96(17):1880-8. doi: 10.1016/j.biortech.2005.01.032.

DOI:10.1016/j.biortech.2005.01.032
PMID:16084367
Abstract

The filtrate obtained by interacting a known amount of rice husk with deionised, Milli-Q water was assessed as a carbon source and nutrient medium for the growth of Desulfotomaculum nigrificans, a typical sulfate-reducing bacterium. The filtrate contained essential growth constituents such as magnesium, potassium, phosphorous apart from calcium, sodium, chloride and sulfate ions. Based on the 1H and 13C NMR characterization studies, the organic composition of the components dissolved from the rice husk, was found to be: (i) 66% lignocellulosic material, (ii) 24% xylose+arabinose and (iii) 10% galactose. The growth studies indicated a 15-fold increase in the bacterial cell number in about 20 days. Nearly 81% and 66% reduction in sulfate concentration could be achieved in about 28 days, from the solutions containing initial sulfate concentrations of 550 mg/l and 1200 mg/l respectively. In both the cases studied, the iron concentration could be reduced by over 85%.

摘要

将已知量的稻壳与去离子超纯水相互作用得到的滤液,被评估为一种典型的硫酸盐还原菌——黑色脱硫肠状菌生长的碳源和营养培养基。该滤液除含有钙、钠、氯离子和硫酸根离子外,还含有镁、钾、磷等基本生长成分。基于1H和13C核磁共振表征研究,发现从稻壳中溶解的成分的有机组成如下:(i) 66%的木质纤维素材料,(ii) 24%的木糖+阿拉伯糖,(iii) 10%的半乳糖。生长研究表明,在大约20天内细菌细胞数量增加了15倍。分别从初始硫酸盐浓度为550 mg/l和1200 mg/l的溶液中,在大约28天内可以实现硫酸盐浓度分别降低近81%和66%。在所研究的两种情况下,铁浓度都可以降低超过85%。

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