Ramirez James A, Guitart Joan, Rao M Sambasiva, Diaz Leslie K
Department of Dermatology, Northwestern University Feinberg School of Medicine, Chicago, IL 60611, USA.
Ann Diagn Pathol. 2005 Aug;9(4):185-8. doi: 10.1016/j.anndiagpath.2005.04.018.
Progression through the cell cycle is controlled by cyclins, cyclin-dependent kinases, and cyclin-dependent kinase inhibitory proteins. The role of cyclin D1 in the development and progression of melanomas is controversial. The goal of this study is to evaluate the role of cyclin D1 in benign and malignant melanocytic lesions of the skin.
A total of 126 pigmented lesions of the skin including compound nevi (21), intradermal nevi (18), melanoma in situ (28), primary invasive melanomas (30), and metastatic melanoma (29) were evaluated for cyclin D1 expression. The following tiered system was used for scoring: 0% nuclear staining (score 0), 1% to 19% nuclear staining (score 1), 20% to 49% nuclear staining (score 2), and 50% or greater nuclear staining (score 3).
Average scores were significantly higher for primary melanomas compared with nevi and for in situ melanomas compared with primary invasive melanomas. The average score for metastatic melanomas was not significantly different compared with primary invasive melanomas. Scores for primary invasive melanomas did not correlate with depth of invasion or presence of metastases. Compound nevi exhibited a slightly higher level of cyclin D1 expression compared with intradermal nevi.
Although primary melanomas show a higher level of cyclin D1 expression compared with nevi, cyclin D1 appears to have little role in development of a metastatic phenotype. It is not clear why lesions localized near the dermal-epidermal junction express higher levels of cyclin D1. Further studies are indicated to ascertain the biologic role and practical utility of cyclin D1 in melanocytic lesions of the skin.
细胞周期的进程由细胞周期蛋白、细胞周期蛋白依赖性激酶和细胞周期蛋白依赖性激酶抑制蛋白控制。细胞周期蛋白D1在黑色素瘤发生发展中的作用存在争议。本研究的目的是评估细胞周期蛋白D1在皮肤良性和恶性黑素细胞性病变中的作用。
对总共126例皮肤色素沉着病变进行细胞周期蛋白D1表达评估，这些病变包括复合痣（21例）、皮内痣（18例）、原位黑色素瘤（28例）、原发性浸润性黑色素瘤（30例）和转移性黑色素瘤（29例）。采用以下分级系统进行评分：细胞核染色0%（评分0）、细胞核染色1%至19%（评分1）、细胞核染色20%至49%（评分2）以及细胞核染色50%或更高（评分3）。
与痣相比，原发性黑色素瘤的平均评分显著更高；与原发性浸润性黑色素瘤相比，原位黑色素瘤的平均评分显著更高。转移性黑色素瘤的平均评分与原发性浸润性黑色素瘤相比无显著差异。原发性浸润性黑色素瘤的评分与浸润深度或转移情况无关。与皮内痣相比，复合痣的细胞周期蛋白D1表达水平略高。
虽然与痣相比，原发性黑色素瘤显示出更高水平的细胞周期蛋白D1表达，但细胞周期蛋白D1似乎在转移表型的发展中作用不大。尚不清楚为什么位于真皮 - 表皮交界处附近的病变表达更高水平的细胞周期蛋白D1。需要进一步研究以确定细胞周期蛋白D1在皮肤黑素细胞性病变中的生物学作用和实际应用价值。
