Wei Jian-Jun, Chiriboga Luis, Mittal Khush
Department of Pathology, New York University School of Medicine, New York, New York, USA.
Fertil Steril. 2005 Aug;84(2):474-84. doi: 10.1016/j.fertnstert.2005.01.142.
To use tissue microarray in combination with dendrogram cluster analysis to characterize some potential tumorigenic factors in association with tumor size and sex steroid hormone status in uterine leiomyomata.
Expression analysis of 21 selected potential tumorigenic factors in 60 patients with uterine leiomyomata.
University clinical research laboratory.
PATIENT(S): Hysterectomy specimens from 60 patients with uterine leiomyomata of different ages and tumor sizes.
INTERVENTION(S): Tissue cores from normal myometrium and leiomyomata were examined by immunohistochemistry.
MAIN OUTCOME MEASURE(S): Semiquantitative immunointensity was scored and analyzed by net gain and loss between normal myometrium and leiomyomata and integrated into dendrogram cluster tree view.
RESULT(S): We found that upregulation of estrogen and progesterone receptors was reverse associated with tumor size. Upregulation of some factors (HMGA2, sex steroid receptor cofactors, proteins in insulin pathway, and CD24) were identified in a group of patients in their later forties, were associated with large fibroids, and were weakly affected by the SSH status (illustrated by endometrial phases and menopause). Downregulation of tuberin and glucocorticoid receptor was mostly isolated in a second group of women at their late reproductive age.
CONCLUSION(S): Analyses of the sex steroid hormone receptors and the nonsex steroid hormone factors in leiomyomata and the matched myometrium showed different expression patterns in different tumor sizes and patients' ages. A group of patients in their late forties with the larger leiomyomata contributes largely by upregulation of nonsex steroid hormone factors. Adenomyosis is a protective factor preventing large leiomyomata.
联合使用组织微阵列和树形图聚类分析来鉴定一些与子宫肌瘤的肿瘤大小及性甾体激素状态相关的潜在致瘤因素。
对60例子宫肌瘤患者的21种选定潜在致瘤因素进行表达分析。
大学临床研究实验室。
60例不同年龄和肿瘤大小的子宫肌瘤患者的子宫切除标本。
通过免疫组织化学检查正常子宫肌层和肌瘤的组织芯。
对免疫强度进行半定量评分，并分析正常子宫肌层和肌瘤之间的净增减情况，并整合到树形图聚类树状图中。
我们发现雌激素和孕激素受体的上调与肿瘤大小呈负相关。在一组四十多岁后期的患者中，发现一些因素（HMGA2、性甾体受体辅因子、胰岛素途径中的蛋白质和CD24）上调，这些因素与大肌瘤相关，并且受性甾体激素状态（以内膜分期和绝经为例）的影响较弱。结节性硬化蛋白和糖皮质激素受体的下调大多出现在第二组生殖后期的女性中。
对肌瘤和匹配的子宫肌层中的性甾体激素受体和非性甾体激素因素的分析显示，在不同的肿瘤大小和患者年龄中存在不同的表达模式。一组四十多岁后期患有较大肌瘤的患者主要是通过非性甾体激素因素的上调起作用。子宫腺肌病是预防大肌瘤的保护因素。
