Wei Jian-Jun, Zhang Xing-Min, Chiriboga Luis, Yee Herman, Perle Mary A, Mittal Khush
Department of Pathology, Bellevue Hospital, New York, New York, USA.
Fertil Steril. 2006 Jan;85(1):179-87. doi: 10.1016/j.fertnstert.2005.07.1294.
To evaluate the growth pattern of the large uterine leiomyomata (ULM), we examined the spatial gene distributions, vessel density, proliferative activity, and hyaline degeneration.
Tissue sections from three-dimensional large ULM, matched myometrium, and small ULM were collected and microarrayed. The spatial difference of the tumor activity was mapped in large ULM.
University clinical research laboratory.
PATIENT(S): Hysterectomy specimens from 7 patients with large (>10 cm) ULM and 3 patients with large (>10 cm) uterine leiomyosarcomas.
INTERVENTION(S): Tissue microarray analysis by the immunohistochemistry.
MAIN OUTCOME MEASURE(S): Selected gene products, vessel density, and the percentage of hyaline degeneration were all scored in tissue cores/sections of large and small ULM against matched myometrium.
RESULT(S): We found that there was a spherical spatial difference of the tumor activities in large ULM. The tumor region next to the periphery, the most biologically active zone, demonstrated higher levels of gene expression, a higher density of vessels, a higher proliferative rate and a lower level of hyaline degeneration. The large ULM have higher levels of gene products (except for estrogen and progesterone receptors) than small ULM.
CONCLUSION(S): In comparison of the spatial patterns of the gene activity between the large ULM and the large uterine leiomyosarcoma, the large ULM illustrate a growth pattern of nutritional dependence.
为评估大型子宫平滑肌瘤（ULM）的生长模式，我们检测了空间基因分布、血管密度、增殖活性和透明变性。
收集三维大型ULM、匹配的子宫肌层和小型ULM的组织切片并制作微阵列。绘制大型ULM中肿瘤活性的空间差异图。
大学临床研究实验室。
7例大型（>10 cm）ULM患者和3例大型（>10 cm）子宫平滑肌肉瘤患者的子宫切除标本。
通过免疫组织化学进行组织微阵列分析。
在大型和小型ULM的组织芯/切片中，针对匹配的子宫肌层，对选定的基因产物、血管密度和透明变性百分比进行评分。
我们发现大型ULM中肿瘤活性存在球形空间差异。周边相邻的肿瘤区域是最具生物活性的区域，其基因表达水平更高、血管密度更高、增殖率更高且透明变性水平更低。大型ULM的基因产物水平（雌激素和孕激素受体除外）高于小型ULM。
比较大型ULM和大型子宫平滑肌肉瘤之间基因活性的空间模式，大型ULM呈现出营养依赖的生长模式。
