Horiuchi Reii, Akahata Wataru, Kuwata Takeo, Enose Yoshimi, Ido Eiji, Suzuki Hajime, Miyake Ariko, Saito Naoki, Ibuki Kentaro, Goto Toshiyuki, Miura Tomoyuki, Hayami Masanori
Laboratory of Primate Model, Experimental Research Center for Infectious Diseases, Institute for Virus Research, Kyoto University, 53 Shogoin-kawahara-cho, Sakyo-ku, Kyoto 606-8507, Japan.
Vaccine. 2006 Apr 24;24(17):3677-85. doi: 10.1016/j.vaccine.2005.07.006. Epub 2005 Jul 20.
We previously reported that a mutant full-sized plasmid DNA vaccine regime in macaques was effective against a homologous challenge [Akahata W, Ido E, Shimada T, Katsuyama K, Yamamoto H, Uesaka H, et al. DNA vaccination of macaques by a full genome HIV-1 plasmid which produces non-infectious virus particles. Virology 2000;275:116-24; Akahata W, Ido E, Akiyama H, Uesaka H, Enose Y, Horiuchi R, et al. DNA vaccination of macaques by a full genome SHIV-1 plasmid that produces non-infectious virus particles. J Gen Virol 2003;84:2237-44]. In this study, to evaluate the DNA vaccination regime against a heterologous challenge, a novel plasmid named pSHIV-ZF1IL-2 was constructed. Four monkeys were intramuscularly and intradermally injected four times with the pSHIV-ZF1IL-2. Vaccinated monkeys were intravenously challenged with a highly pathogenic, heterologous SHIV at 11 weeks post vaccination. All the vaccinated monkeys suppressed the challenge virus rapidly under the detectable level by 16 weeks post challenge. One vaccinated monkey was protected from a loss of CD4+ T cells. These results suggest pSHIV-ZF1*IL-2 alone seems partially effective even against a challenge with a heterologous, pathogenic virus.
我们之前报道过，猕猴中的一种突变型全长质粒DNA疫苗方案对同源攻击有效[赤羽田W，井藤E，岛田T，胜山K，山本H，上坂H等。用产生非感染性病毒颗粒的全基因组HIV-1质粒对猕猴进行DNA疫苗接种。病毒学2000；275：116 - 24；赤羽田W，井藤E，秋山H，上坂H，榎濑Y，堀内R等。用产生非感染性病毒颗粒的全基因组SHIV-1质粒对猕猴进行DNA疫苗接种。普通病毒学杂志2003；84：2237 - 44]。在本研究中，为了评估DNA疫苗方案对异源攻击的效果，构建了一种名为pSHIV-ZF1IL-2的新型质粒。4只猕猴通过肌肉注射和皮内注射接受4次pSHIV-ZFIL-2。接种疫苗的猕猴在接种后11周静脉注射高致病性异源SHIV进行攻击。所有接种疫苗的猕猴在攻击后16周内迅速将攻击病毒抑制到检测水平以下。1只接种疫苗的猕猴免受CD4+T细胞损失的影响。这些结果表明，单独使用pSHIV-ZF1*IL-2即使对异源致病性病毒的攻击似乎也有部分效果。
