Xu Ya-jun, Zhang Guo-qing, Li Yong
Department of Nutrition & Food Hygiene, Peking University School of Public Health, Beijing 100083, China.
Beijing Da Xue Xue Bao Yi Xue Ban. 2005 Aug 18;37(4):346-50.
To probe the effect of prenatal ethanol exposure on the mitochondrial protein expression in fetal mouse cerebrum.
Pregnant CD-1 mice were given 5.0 g/kg ethanol intragastrically from pregnant days (PD) 6-15. Fetal cerebral mitochondria were isolated on PD 18. The overall mitochondrial protein was applied to two-dimensional gel electrophoresis. Differentially expressed protein spots were cut off and identified by matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry. Activities of respiratory chain complex IV (72.3%+/-4.6% of control) and ATP synthase, and ATP content in the fetal cerebral cells were measured.
Expressions of some constructional and functional proteins were altered by in utero ethanol exposure. Activities of respiratory chain complex IV and ATP synthase (80.3%+/-5.1% of control) were both decreased. ATP content (67.9%+/-3.9% of control) in the cerebral cells was reduced in the in utero ethanol exposure fetuses.
In utero ethanol exposure affects the mitochondrial protein expression of fetal mouse cerebrum, even in the tissue without obvious morphological malformations, which may be a possible mechanism of behavioral defects found in children with prenatal ethanol exposure.
探讨孕期乙醇暴露对胎鼠大脑线粒体蛋白表达的影响。
将妊娠第6至15天的CD-1孕鼠经口给予5.0 g/kg乙醇。在妊娠第18天分离胎鼠脑线粒体。将总的线粒体蛋白进行二维凝胶电泳。切下差异表达的蛋白点，用基质辅助激光解吸电离飞行时间（MALDI-TOF）质谱进行鉴定。测定胎鼠脑细胞中呼吸链复合体IV（对照组的72.3%±4.6%）和ATP合酶的活性以及ATP含量。
宫内乙醇暴露改变了一些结构和功能蛋白的表达。呼吸链复合体IV和ATP合酶的活性（对照组的80.3%±5.1%）均降低。宫内乙醇暴露的胎鼠脑细胞中的ATP含量（对照组的67.9%±3.9%）减少。
宫内乙醇暴露影响胎鼠大脑的线粒体蛋白表达，即使在无明显形态学畸形的组织中也是如此，这可能是孕期乙醇暴露儿童出现行为缺陷的一种可能机制。
