Ragazzini Teresa, Magrini Elisabetta, Cucchi Maria C, Foschini Maria P, Eusebi V
Department of Oncology, Section of Pathology, University of Bologna at Bellaria Hospital, Bologna, Italy.
Int J Surg Pathol. 2005 Jul;13(3):247-52. doi: 10.1177/106689690501300303.
Thirty-six core breast biopsies from 32 patients were paraffin embedded by use of an automated microwave processor. In addition, a quick immunohistochemical method was used in selected cases. The quality of the hematoxylin and eosin (H&E) slides was very satisfactory, as were also the immunohistochemical stains for ER, PR, and Ki67 when compared to those obtained with the use of a conventional automated immunostainer. The time required to process the tissue to the final H&E stage averaged 2 hours 52 minutes, and the immunohistochemical method required 90 to 100 minutes. This procedure, which we named "fast-track biopsy'' (FTB), is quick enough to be competitive with FNAC (fine-needle aspiration biopsy) in terms of turnaround-times. The superiority of core biopsy over FNA in terms of the morphologic information provided is widely acknowledged, the only major argument currently mentioned in favor of FNAC being the shorter duration of the procedure. With the advent of FTB, it would appear that even this last remaining advantage has been erased.
使用自动微波处理器对32例患者的36份乳腺核心活检标本进行石蜡包埋。此外，在部分病例中采用了快速免疫组织化学方法。苏木精和伊红（H&E）染色切片的质量非常令人满意，与使用传统自动免疫染色仪获得的结果相比，雌激素受体（ER）、孕激素受体（PR）和Ki67的免疫组织化学染色结果同样令人满意。将组织处理至最终H&E阶段平均需要2小时52分钟，免疫组织化学方法需要90至100分钟。我们将此程序命名为“快速活检”（FTB），其速度足够快，在周转时间方面足以与细针穿刺抽吸活检（FNAC）相竞争。在提供的形态学信息方面，核心活检优于细针穿刺抽吸活检，这一点已得到广泛认可，目前提及的支持FNAC的唯一主要论据是该操作持续时间较短。随着FTB的出现，似乎这最后一个剩余优势也已不复存在。
