日本A组轮状病毒G型的分布变化及G9型的基因分析

Changing distribution of group A rotavirus G-types and genetic analysis of G9 circulating in Japan.

作者信息

Yoshinaga M, Phan T G, Nguyen T A, Yan H, Yagyu F, Okitsu S, Müller W E G, Ushijima H

机构信息

Department of Developmental Medical Sciences, Institute of International Health, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.

出版信息

Arch Virol. 2006 Jan;151(1):183-92. doi: 10.1007/s00705-005-0604-z. Epub 2005 Aug 8.

DOI:10.1007/s00705-005-0604-z

PMID:16086098

Abstract

A total of 1,797 fecal specimens from infants and children with acute gastroenteritis in Japan from July 2000 to June 2003 were tested for group A rotavirus by ELISA, RT-PCR, RNA-PAGE and latex agglutination methods. Of these, 439 were found to be positive for group A rotavirus and this presented 24.4%. In 2000-2001, G1 was the most prevalent (45.5%) followed by G2 (32.5%), G3 (12.3%), G9 (5.9%) and G4 (2.6%). However, G2 was found predominant with 40% in the following year (2001-2002). Interestingly, G9 had a rapid increase of infection up to 17.8%. In 2002-2003, G3 dominated over other G-types with 34%. Another interesting feature of the study was the demonstration of great genetic diversity among G9 strains in Japan. Worth of note was the first prevalence pattern of rotavirus G-types with an increase of G2, G3 as well as G9 and a decrease of G1 during the 20 year-survey of rotavirus infection in Japan.

摘要

2000年7月至2003年6月期间，对日本1797份急性胃肠炎婴幼儿粪便标本采用ELISA、RT-PCR、RNA-PAGE和乳胶凝集法检测A组轮状病毒。其中，439份A组轮状病毒检测呈阳性，占比24.4%。2000 - 2001年，G1型最为常见（45.5%），其次是G2型（32.5%）、G3型（12.3%）、G9型（5.9%）和G4型（2.6%）。然而，次年（2001 - 2002年）G2型占主导，为40%。有趣的是，G9型感染率迅速上升至17.8%。2002 - 2003年，G3型以34%的占比超过其他G型。该研究的另一个有趣特点是，在日本G9株中显示出巨大的遗传多样性。值得注意的是，在日本20年的轮状病毒感染调查中，轮状病毒G型首次出现G2、G3以及G9型增加而G1型减少的流行模式。