Zhou Y, Supawadee J, Khamwan C, Tonusin S, Peerakome S, Kim B, Kaneshi K, Ueda Y, Nakaya S, Akatani K, Maneekarn N, Ushijima H
Department of Developmental Medical Sciences, Institute of International Health, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
J Med Virol. 2001 Nov;65(3):619-28.
Serotyping of human rotavirus was conducted in 396 Japanese and 100 Thai rotavirus-positive fecal specimens collected from 1995 to 1997. Serotype G9 was found to be the third most common serotype with frequency of 16.2% in Thailand from 1996 to 1997. It was also detected in Japan with a low frequency (0.7%) in this year. The genetic analyses of VP4 and NSP4 genes of these G9 strains showed that 1 strain from Japan possessed P[8] genotype and NSP4 Wa-group with long electropherotype (e-type). In contrast, 5 strains from Thailand belonged to P[6] and 1 strain belonged to P[4]. All of the Thai strains were in the NSP4 KUN-group with a short e-type. Sequence analysis of their VP7 gene revealed that there was the highest homology among fecal G9 strains (> 96.3%, amino acid identity) and a relatively high degree of homology to standard viruses, F45 from Japan (95.4-96.3%, amino acid identity) and 116E from India (92-92.3%, amino acid identity). However, immunological analysis using G9 specific monoclonal antibodies (Mabs) against VP7 protein showed that the G9 strains isolated from the two countries had different antigenic specificity. It was confirmed further by intraserotypical phylogenetic analysis of VP7 amino acid. These results indicated that the prevalence of G9 rotavirus in 1996-1997 in Thailand was relative to the continuing recent emergence of it on a worldwide basis, while the Japanese G9 strain isolated in this survey was identified to have progenitors common to the F45 strain that was prevalent in 1985 in Japan. Phylogenetic analysis of VP7 amino acid of G1-14 prototype rotavirus showed that the G9 strains were most closely related to the equine G14 rotavirus FI23 strain but G3 strains, interserotypically. These findings suggest that G9 rotaviruses might be divided into two or more subtypes.
对1995年至1997年收集的396份日本人和100份泰国人轮状病毒阳性粪便标本进行了人轮状病毒血清分型。血清型G9被发现是第三常见的血清型，1996年至1997年在泰国的出现频率为16.2%。同年在日本也有低频率（0.7%）的检测到。对这些G9毒株的VP4和NSP4基因进行遗传分析表明，来自日本的1株具有P[8]基因型和NSP4 Wa组，电泳型长（e型）。相比之下，来自泰国的5株属于P[6]，1株属于P[4]。所有泰国毒株都属于NSP4 KUN组，e型短。对其VP7基因的序列分析表明，粪便G9毒株之间具有最高的同源性（>96.3%，氨基酸同一性），与日本的标准病毒F45（95.4-96.3%，氨基酸同一性）和印度的116E（92-92.3%，氨基酸同一性）具有相对较高的同源性。然而，使用针对VP7蛋白的G9特异性单克隆抗体（Mab）进行的免疫分析表明，从两国分离的G9毒株具有不同的抗原特异性。VP7氨基酸的血清型内系统发育分析进一步证实了这一点。这些结果表明，1996 - 1997年泰国G9轮状病毒的流行与它在全球范围内最近持续出现有关，而本次调查中分离的日本G9毒株被确定具有与1985年在日本流行的F45毒株共同的祖先。G1 - 14原型轮状病毒VP7氨基酸的系统发育分析表明，G9毒株与马G14轮状病毒FI23株关系最为密切，但在血清型间与G3毒株关系密切。这些发现表明，G9轮状病毒可能分为两个或更多亚型。
