Rico María José, Matar Pablo, Gervasoni Silvia I, Bonfil R Daniel, Calcaterra Nora, Scharovsky O Graciela
Instituto de Genética Experimental, Universidad National de Rosario, Rosario, Argentina.
Clin Exp Metastasis. 2005;22(2):127-35. doi: 10.1007/s10585-005-5140-4.
Interleukin 10 (IL-10) is a Th2 anti-inflammatory cytokine that participates in the regulation of the immune response at several levels. Its production has been implicated in the immunosuppression frequently observed in tumor bearing hosts. The broad spectrum of IL-10 biologic activities is mediated by its binding to its cognate receptor (IL-10R). We have already demonstrated the overproduction of IL-10 by B-cell lymphoma tumor bearing rats and, also, that IL-10 could act as a growth factor for metastatic cells. Considering the importance to unravel each feature of the complex biology of metastasis, the goal of the present study was to investigate the expression of IL-10 receptor (IL-10R), at mRNA and protein level, in primary tumor and metastatic cells from a rat B-cell lymphoma, along with the production of IL-10 by both tumor cell types. Our results indicate that IL-10, besides its immunoregulatory effect, would act as an autocrine growth factor for cells with metastatic phenotype. Also, the up-regulation of IL-10 and IL-10R expression would be part of the transition from primary tumor to the metastatic phenotype.
白细胞介素10（IL-10）是一种Th2抗炎细胞因子，在多个水平参与免疫反应的调节。其产生与荷瘤宿主中经常观察到的免疫抑制有关。IL-10广泛的生物学活性是通过其与同源受体（IL-10R）结合来介导的。我们已经证明荷B细胞淋巴瘤大鼠会过度产生IL-10，并且IL-10可作为转移细胞的生长因子。考虑到阐明转移复杂生物学的每个特征的重要性，本研究的目的是在mRNA和蛋白质水平上研究IL-10受体（IL-10R）在大鼠B细胞淋巴瘤原发肿瘤和转移细胞中的表达，以及这两种肿瘤细胞类型产生IL-10的情况。我们的结果表明，IL-10除了具有免疫调节作用外，还可作为具有转移表型细胞的自分泌生长因子。此外，IL-10和IL-10R表达的上调将是从原发肿瘤向转移表型转变的一部分。
