Zhang Hong-bo, Lai Jiang-hua, Li Sheng-bin
The Key Laboratory of National Ministry of Health for Forensic Sciences, Key Laboratory of Environment and Genes Related to Diseases, Ministry of Education, Xi'an Jiaotong University, Xi'an, Shaanxi, 710061 PR China.
Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2005 Aug;22(4):464-6.
To investigate HLA-A, -B and -DRB1 allele and HLA-A-B, B-DRB1, A-B-DRB1 haplotype frequencies in the northwest Chinese Han population.
The authors investigated the HLA-A, -B, -DRB1 allele and haplotype in a northwest Chinese Han population based on 62 families and 101 individuals by use of PCR-sequence specific oligonucleotide probes(PCR-SSOP) DNA typing methods.
Fifteen alleles for the locus HLA-A, 28 alleles for the HLA-B locus and 13 alleles for the HLA-DRB1 were detected. The results showed that the most frequent HLA alleles found were A02 (0.3244), B13 (0.1200), and DRB115 (0.1400). Allele frequencies of more than 10% for HLA antigens were A02, A11, A24, B13, B15, B40, DRB104, DRB107, DRB109, DRB115. In the analysis of HLA haplotypes, 122 kinds of HLA-A-B haplotypes and 147 kinds HLA-B-DRB1 haplotypes were found. Two hundred and seventy-eight kinds of HLA-A-B-DRB1 haplotypes were found, comprising 61.78%(278/450) of total theoretical haplotypes. Eighty-three kinds of HLA-A-B-DRB1 haplotypes were shown to have at least two same haplotypes, comprising 18.44%(83/450) of total theoretical haplotypes. The most common HLA-A-B-DRB1 haplotypes were A30-B13-DRB107, A02-B46-DRB109, A01-B37-DRB110, A24-B15-DRB115, A02-B46-DRB108, A33-B58-DRB1*03.
The data can be used for the estimation of the probability of finding haplotypically identical, related or unrelated bone marrow donor for an individual patient, and forensic and paternity tests to estimate the frequency of a DNA profile or anthropologic research.
研究中国西北汉族人群中HLA - A、- B和 - DRB1等位基因以及HLA - A - B、B - DRB1、A - B - DRB1单倍型频率。
作者采用聚合酶链反应 - 序列特异性寡核苷酸探针（PCR - SSOP）DNA分型方法，对中国西北汉族人群中62个家庭的101名个体进行了HLA - A、- B、- DRB1等位基因和单倍型的研究。
检测到HLA - A位点有15个等位基因，HLA - B位点有28个等位基因，HLA - DRB1位点有13个等位基因。结果显示，最常见的HLA等位基因是A02（0.3244）、B13（0.1200）和DRB115（0.1400）。HLA抗原频率超过10%的有A02、A11、A24、B13、B15、B40、DRB104、DRB107、DRB109、DRB115。在HLA单倍型分析中，发现了122种HLA - A - B单倍型和147种HLA - B - DRB1单倍型。发现了278种HLA - A - B - DRB1单倍型，占理论单倍型总数的61.78%（278/450）。83种HLA - A - B - DRB1单倍型显示至少有两种相同单倍型，占理论单倍型总数的18.44%（83/450）。最常见的HLA - A - B - DRB1单倍型是A30 - B13 - DRB107、A02 - B46 - DRB109、A01 - B37 - DRB110、A24 - B15 - DRB115、A02 - B46 - DRB108、A33 - B58 - DRB1*03。
这些数据可用于估计为个体患者找到单倍型相同、相关或无关骨髓供体的概率，以及用于法医和亲子鉴定以估计DNA图谱频率或人类学研究。
