Bottier D, Caroff B, Goldschmidt P, Mbopi Keou F X, Cotigny S, Matta F M, Longuet M F, Bélec L
Service de Microbiologie, Hôpital Broussais, Paris, France.
Pathol Biol (Paris). 1992 Mar;40(3):223-9.
Among 770 Western blots for HIV-1 confirmation on sera from subjects at high risk for HIV infection, 4.3% (33 cases) were indeterminate. Isolated, stable, reproducible anti-gp 160 reactivity, highly suggestive of a nonspecific reaction, was found in 16% of cases. There were three other probably nonspecific patterns with anti-gp 160 and either anti-gp 41 or anti-gp 120 reactivities and thin, atypical bands. Two patterns with anti-p24 and either anti-gp 160 or anti-gp 120 reactivities were consistent with HIV-1 seroconversion. Reactivity directed solely against gag products was seen in 18% of cases. The repeat test, performed in 16 cases, showed an identical pattern in 3 cases, a modified pattern in 3 cases, negative results in 9 cases, and seroconversion in 1 case. No case of HIV-2 infection was detected. Indeterminate Western blot results reflect nonspecific reactivity in most instances but should nevertheless lead to the exclusion of technical artefacts, seroconversion, and HIV-2 infection.
在对770份来自HIV感染高危人群血清进行HIV-1确认的蛋白质印迹检测中，4.3%（33例）结果不确定。在16%的病例中发现了孤立、稳定、可重复的抗gp160反应性，高度提示为非特异性反应。还有另外三种可能的非特异性模式，表现为抗gp160与抗gp41或抗gp120反应性以及细的、非典型条带。两种抗p24与抗gp160或抗gp120反应性的模式与HIV-1血清转化一致。仅针对gag产物的反应性在18%的病例中可见。对16例进行了重复检测，3例结果相同，3例模式改变，9例结果为阴性，1例发生血清转化。未检测到HIV-2感染病例。不确定的蛋白质印迹结果在大多数情况下反映了非特异性反应性，但仍应排除技术假象、血清转化和HIV-2感染。
