Bressan Alberto, Clair Denis, Sémétey Olivier, Boudon-Padieu Elisabeth
Biologie et Ecologie des Phytoplasmes, UMR Plante Microbe Environnement, INRA, BP 86510, 21065 Dijon Cedex, France.
J Invertebr Pathol. 2005 Jun;89(2):144-9. doi: 10.1016/j.jip.2005.03.001. Epub 2005 Apr 7.
We have investigated the influence on longevity and fecundity of Flavescence dorée phytoplasma (FDP), the agent of a grapevine yellows disease, in the experimental vector Euscelidius variegatus Kirschbaum. Late instar nymphs were exposed to one or the other of two strains of FDP (FD92 and FD2000) by feeding on infected broad bean (Vicia faba L.) or on healthy broad bean or maize (Zea mays L.) for an acquisition access period of 13 days. Detection of FDP in individual insects was done with PCR assays and revealed that almost all exposed leafhoppers had acquired FDP, for both FD92 and FD2000 strains. FDP infection significantly reduced the life span of males and females (ANOVA of the quartiles of survival distribution and Weibull scale parameter). FDP-exposed females produced significantly fewer nymphs. The two FDP strains had similar effects on reduction of survival and fecundity of leafhoppers. There was no significant differences in longevity of E. variegatus males exposed to FD broad bean than held on healthy broad bean or maize, but female survival and fecundity were reduced when they fed on maize versus healthy broad bean.
我们研究了葡萄黄化病病原体——金黄叶蝉植原体（FDP）对实验载体杂色片角叶蝉（Euscelidius variegatus Kirschbaum）寿命和繁殖力的影响。通过在感染的蚕豆（Vicia faba L.）、健康蚕豆或玉米（Zea mays L.）上取食13天的获取期，将末龄若虫暴露于两种FDP菌株（FD92和FD2000）中的一种。通过PCR检测单个昆虫体内的FDP，结果显示，对于FD92和FD2000菌株，几乎所有暴露的叶蝉都感染了FDP。FDP感染显著缩短了雄性和雌性的寿命（生存分布四分位数和威布尔尺度参数的方差分析）。暴露于FDP的雌性叶蝉产卵显著减少。两种FDP菌株对叶蝉存活率和繁殖力的降低具有相似的影响。暴露于FD蚕豆上的杂色片角叶蝉雄性与饲养在健康蚕豆或玉米上的雄性相比，寿命没有显著差异，但当雌性取食玉米而非健康蚕豆时，其存活率和繁殖力会降低。
