用于防治萝卜蝇（Delia radicum (L.)）幼虫的昆虫病原真菌的实验室生物测定

Laboratory bioassays of entomopathogenic fungi for control of Delia radicum (L.) larvae.

作者信息

Bruck Denny J, Snelling Jane E, Dreves Amy J, Jaronski Stefan T

机构信息

USDA-ARS, Horticultural Crops Research Laboratory, 3420 NW Orchard Avenue, Corvallis, OR 97330, USA.

出版信息

J Invertebr Pathol. 2005 Jun;89(2):179-83. doi: 10.1016/j.jip.2005.02.007. Epub 2005 Apr 7.

DOI:10.1016/j.jip.2005.02.007

PMID:16087004

Abstract

Laboratory soil bioassays were performed at economic field rates for in-furrow (3.85 x 10(6)spores/g dry soil) and broadcast (3.85 x 10(5)spores/g dry soil) applications with three isolates of Metarhizium anisopliae (F52, ATCC62176, and ARSEF5520) and one isolate of Beauveria bassiana (GHA). All isolates tested were infective to second instar Delia radicum (L.). The conditionally registered M. anisopliae isolate (F52) performed best killing an average of 85 and 72% of D. radicum larvae at the high and low concentration, respectively. The mean LC50 and LC95 of F52 against second instar D. radicum was 2.7 x 10(6) and 1.8 x 10(8)spores/g dry soil, respectively. The use of F52 in an integrated management program is discussed.

摘要

采用绿僵菌（F52、ATCC62176和ARSEF5520）的三个分离株和球孢白僵菌（GHA）的一个分离株，以经济田间用量进行沟施（3.85×10⁶孢子/克干土）和撒施（3.85×10⁵孢子/克干土）的实验室土壤生物测定。所有测试的分离株均对萝卜地种蝇二龄幼虫具有感染力。有条件登记的绿僵菌分离株（F52）表现最佳，在高浓度和低浓度下分别平均杀死85%和72%的萝卜地种蝇幼虫。F52对萝卜地种蝇二龄幼虫的平均LC50和LC95分别为2.7×10⁶和1.8×10⁸孢子/克干土。文中还讨论了在综合管理计划中使用F52的情况。