Diestra Elia, Solé Antonio, Martí Mercedes, Garcia de Oteyza Tirso, Grimalt Joan O, Esteve Isabel
Department of Genetics and Microbiology, Autonomous University of Barcelona, 08193 Bellaterra, Spain.
Scanning. 2005 Jul-Aug;27(4):176-80. doi: 10.1002/sca.4950270404.
A consortium of microorganisms with the capacity to degrade crude oil has been characterized by means of confocal laser scanning microscopy (CLSM), transmission electron microscopy (TEM), and scanning electron microscopy (SEM). The analysis using CLSM shows that Microcoleus chthonoplastes is the dominant organism in the consortium. This cyanobacterium forms long filaments that group together in bundles inside a mucopolysaccharide sheath. Scanning electron microscopy and transmission electron microscopy have allowed us to demonstrate that this cyanobacterium forms a consortium primarily with three morphotypes of the heterotrophic microorganisms found in the Microcoleus chthonoplastes sheath. The optimal growth of Microcoleus consortium was obtained in presence of light and crude oil, and under anaerobic conditions. When grown in agar plate, only one type of colony (green and filamentous) was observed.
一个具有降解原油能力的微生物群落已通过共聚焦激光扫描显微镜（CLSM）、透射电子显微镜（TEM）和扫描电子显微镜（SEM）进行了表征。使用CLSM的分析表明，鞘丝藻是该群落中的优势生物。这种蓝细菌形成长丝，在粘多糖鞘内成束聚集在一起。扫描电子显微镜和透射电子显微镜使我们能够证明，这种蓝细菌主要与在鞘丝藻鞘中发现的三种异养微生物形态型形成一个群落。鞘丝藻群落的最佳生长是在有光和原油存在且厌氧的条件下获得的。当在琼脂平板上生长时，只观察到一种类型的菌落（绿色且呈丝状）。
