Xie Cheng-yao, Wang Liang, Liu Yang, Liu Nan, Qiu Xue-shan, Wang En-hua
Department of Pathology, College of Basic Medical Science, China Medical University, Shenyang 110001, China.
Zhonghua Bing Li Xue Za Zhi. 2005 Apr;34(4):206-10.
To investigate the expression of p120(ctn) in non-small cell lung cancer (NSCLC) and its correlation with the clinical and pathologic parameters.
Immunohistochemistry (S-P method) was used to detect the expression of p120(ctn) in 143 NSCLC cases. The variation of protein expression was further analyzed in 36 cases by Western blot. The correlation with clinical and pathologic parameters was studied.
Immunohistochemically, normal bronchial cells showed membranous expression for p120(ctn), while NSCLC was characterized by cytoplasmic or diminished membranous staining. The rate of abnormal p120(ctn) expression was 79.7% (114/143). There was a significant correlation between abnormal expression of p120(ctn) and tumor differentiation, clinical stage, lymph node metastasis and poor prognosis (< 0.05), but not histologic typing. Western blot showed that the total amount of p120(ctn) in normal bronchial cells was significantly higher than that in NSCLC. The p120(ctn) isoform 1 (120,000) and isoform 3 (100,000) were expressed in normal lung tissue, while there was a reduced expression or absence of isoform 1 in NSCLC.
The expression of p120(ctn) is abnormal in NSCLC; p120(ctn) may serve as a useful prognostic marker for NSCLC.
探讨p120（连环蛋白）在非小细胞肺癌（NSCLC）中的表达及其与临床病理参数的相关性。
采用免疫组织化学（S-P法）检测143例NSCLC患者中p120（连环蛋白）的表达。通过蛋白质印迹法进一步分析36例患者蛋白质表达的变化情况。研究其与临床病理参数的相关性。
免疫组织化学结果显示，正常支气管细胞中p120（连环蛋白）呈膜性表达，而NSCLC的特征是细胞质染色或膜性染色减弱。p120（连环蛋白）异常表达率为79.7%（114/143）。p120（连环蛋白）异常表达与肿瘤分化、临床分期、淋巴结转移及预后不良显著相关（<0.05），但与组织学类型无关。蛋白质印迹法显示，正常支气管细胞中p120（连环蛋白）的总量显著高于NSCLC。p120（连环蛋白）同工型1（120,000）和同工型3（100,000）在正常肺组织中表达，而在NSCLC中同工型1表达减少或缺失。
NSCLC中p120（连环蛋白）表达异常；p120（连环蛋白）可能作为NSCLC有用的预后标志物。
