结核分枝杆菌σ因子对耻垢分枝杆菌中ptpAt启动子促进效率的影响。

The Influence of Mycobacterium tuberculosis sigma factors on the promotion efficiency of ptpAt promoter in Mycobacterium smegmatis.

作者信息

Lei Jianqiang, Zhang Hongmei, Wu Chunlan, Wang Xiaolei, Yang Yanping, Zhang Xuelian, Huang Yishu, Wang Honghai

机构信息

State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science Fudan University, Shanghai, 200433, People's Republic of China.

出版信息

Curr Microbiol. 2005 Sep;51(3):141-7. doi: 10.1007/s00284-005-7444-7. Epub 2005 Aug 11.

DOI:10.1007/s00284-005-7444-7

PMID:16091848

Abstract

It was found in a previous study that Mycobacterium tuberculosis protein tyrosine phosphatase ptpAt promoter is a highly active promoter in slow-growing species of mycobacteria, such as M. tuberculosis and M. bovis BCG, but inert in fast-growing mycobacterial species, such as M. smegmatis. This difference is presumed to be due to the differences between sigma factors systems of slow-growing pathogenic mycobacteria and the fast-growing saprophyte M. smegmatis. Therefore, we constructed a series of plasmids, named pOLYG-13x, which can express various M. tuberculosis sigma factors and also contain a P(ptpAt)-gfp reporter gene construct. By inducing different sigma factor genes of M. tuberculosis in M. smegmatis, we were able to explore the influences of various sigma factors on the expression efficiency of the ptpAt promoter. The result show that of the 10 sigma factors evaluated, only sigF and sigL were able to weakly drive the ptpAt promoter in M. smegmatis and other sigma factors were unable to drive the promoter.

摘要

在之前的一项研究中发现，结核分枝杆菌蛋白酪氨酸磷酸酶ptpAt启动子在结核分枝杆菌和牛分枝杆菌卡介苗等生长缓慢的分枝杆菌物种中是一个高活性启动子，但在耻垢分枝杆菌等生长快速的分枝杆菌物种中无活性。这种差异据推测是由于生长缓慢的致病性分枝杆菌与生长快速的腐生菌耻垢分枝杆菌的σ因子系统不同所致。因此，我们构建了一系列名为pOLYG-13x的质粒，其能够表达各种结核分枝杆菌σ因子，并且还包含一个P(ptpAt)-gfp报告基因构建体。通过在耻垢分枝杆菌中诱导结核分枝杆菌的不同σ因子基因，我们能够探究各种σ因子对ptpAt启动子表达效率的影响。结果表明，在所评估的10个σ因子中，只有sigF和sigL能够在耻垢分枝杆菌中微弱地驱动ptpAt启动子，而其他σ因子则无法驱动该启动子。