Barrett B, Borek-Dohalský V, Fejt P, Vaingátová S, Huclová J, Nemec B, Jelínek I
Bioanalytical Laboratory, Cepha s.r.o., 32300, Plzen, Czech Republic.
Anal Bioanal Chem. 2005 Sep;383(2):210-7. doi: 10.1007/s00216-005-0018-5. Epub 2005 Oct 12.
A validated, highly sensitive, and selective HPLC method with MS-MS detection has been developed for quantitative determination of azithromycin (AZI) in human Na2EDTA plasma. Roxithromycin (ROX) was used as internal standard. Human plasma containing AZI and internal standard was ultrafiltered through Centrifree Micropartition devices and the concentration of AZI was determined by isocratic HPLC-MS-MS. Multiple reaction monitoring mode (MRM) was used for MS-MS detection. The calibration plot was linear in the concentration range 2.55-551.43 ng mL(-1). Inter-day and Intra-day precision and accuracy of the proposed method were characterized by R.S.D and percentage deviation, respectively; both were less than 8%. Limit of quantification was 2.55 ng mL(-1). The proposed method was used to determine the pharmacokinetic profile of AZI (250-mg tablets).
已开发出一种经过验证的、高灵敏度且选择性强的采用串联质谱检测的高效液相色谱法，用于定量测定人乙二胺四乙酸二钠（Na2EDTA）血浆中的阿奇霉素（AZI）。罗红霉素（ROX）用作内标。含有AZI和内标的人血浆通过Centrifree微分离装置进行超滤，然后采用等度高效液相色谱 - 串联质谱法测定AZI的浓度。串联质谱检测采用多反应监测模式（MRM）。校准曲线在2.55 - 551.43 ng mL(-1)浓度范围内呈线性。所提出方法的日间和日内精密度及准确度分别用相对标准偏差（R.S.D）和百分偏差表征；两者均小于8%。定量限为2.55 ng mL(-1)。所提出的方法用于测定AZI（250毫克片剂）的药代动力学特征。
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