Panfil B, Chomiczewski K
Zakład Radiobiologii, Wojskowy Instytut Higieny i Epidemiologii, ul. Kozielska 4, 01-163 Warszawa.
Acta Pol Pharm. 1992;49(5-6):31-5.
The effects of 5-fluorouracil (5-FU) on DNA synthesis, mitotic index and cell composition of mouse spleens after 6 Gy X rays irradiation of mice and syngenic bone marrow transplantation were investigated. One dose of 5-FU (100 mg/kg body weight) administered 16 hrs after bone marrow transplantation reduced mitotic index and development of bone marrow grafts, but did not inhibit incorporation of 3H-thymidine into DNA. It correlates with high thymidylate synthase activity previously noted in the same model. Also the increased number of cells from lineage of erythroblasts correlates with high DNA synthesis and thymidylate synthase activity indicating, probably, the presence in those cell lines, the cells with high thymidylate synthase content, resistant to 5-FU.
研究了5-氟尿嘧啶（5-FU）对经6 Gy X射线照射小鼠并进行同基因骨髓移植后小鼠脾脏DNA合成、有丝分裂指数和细胞组成的影响。骨髓移植后16小时给予一剂5-FU（100 mg/kg体重）可降低有丝分裂指数和骨髓移植物的发育，但不抑制3H-胸苷掺入DNA。这与先前在同一模型中观察到的高胸苷酸合成酶活性相关。此外，成红细胞谱系细胞数量的增加与高DNA合成和胸苷酸合成酶活性相关，这可能表明在这些细胞系中存在胸苷酸合成酶含量高且对5-FU耐药的细胞。
