Varga D, Michel I, Patino-Garcia B, Paiss T, Vogel W, Maier C
Department of Human Genetics, University of Ulm, Ulm, Germany.
Cytogenet Genome Res. 2005;111(1):41-5. doi: 10.1159/000085668.
The micronucleus test (MNT) has shown increased micronuclei (MN) frequencies in BRCA associated and sporadic breast cancer patients, Ataxia telangiectasia and Nijmegen Breakage Syndrome patients, demonstrating a common cellular phenotype of increased radiosensitivity. Some genes, causative of these diseases, have also recently been associated with prostate cancer. In order to investigate if prostate cancer exhibits the cellular phenotype of increased radiosensitivity, we performed MNT analysis on 22 sporadic prostate cancer patients and 43 male controls. We determined the baseline MN frequency, in order to see in vivo chromosomal damage without radiation, and induced (after irradiation with 2 Gy) frequency of MN, both in binucleated cells (BNC) obtained from cultured peripheral blood lymphocytes. An automated image analysis system was used to score the MN employing two different classifiers (Classifier A and B) for detection of BNC. The mean baseline frequencies were 48/43 MN/1000 BNC (A/B) for the controls and 42/50 (A/B) for prostate cancer patients. The induced MN frequencies amounted to 107/111 MN/1000 BNC (A/B) for controls and 111/114 MN/1000 BNC (A/B) for prostate cancer patients. The obtained MN frequencies did not result in a statistically significant difference between unselected cases and controls. However, restricting the analysis to young patients (50-60 years, N = 7) and age-matched controls (N = 7) revealed marginally significant higher MN frequencies in patients. We conclude that increased radiosensitivity is not a property of prostate cancer patients in general.
微核试验（MNT）表明，在与乳腺癌相关的散发性乳腺癌患者、共济失调毛细血管扩张症和奈梅亨断裂综合征患者中，微核（MN）频率增加，这表明存在放射敏感性增加这一共同的细胞表型。最近，一些导致这些疾病的基因也与前列腺癌有关。为了研究前列腺癌是否表现出放射敏感性增加的细胞表型，我们对22例散发性前列腺癌患者和43名男性对照进行了MNT分析。我们测定了基线MN频率，以观察无辐射情况下的体内染色体损伤，并测定了从培养的外周血淋巴细胞获得的双核细胞（BNC）经2 Gy照射后的MN诱导频率。使用自动图像分析系统，采用两种不同的分类器（分类器A和B）对BNC进行检测，以对MN进行评分。对照组的平均基线频率为48/43 MN/1000 BNC（A/B）,前列腺癌患者为42/50（A/B）。对照组的MN诱导频率为107/111 MN/1000 BNC（A/B），前列腺癌患者为111/114 MN/1000 BNC（A/B）。在未选择的病例和对照之间，所获得的MN频率没有统计学上的显著差异。然而，将分析限制在年轻患者（50 - 60岁，N = 7）和年龄匹配的对照（N = 7）中，发现患者的MN频率略高。我们得出结论，放射敏感性增加并非前列腺癌患者的普遍特征。
