Ramström Sofia
Department of Biomedicine and Surgery, Division of Clinical Chemistry, University Hospital, Linköping, Sweden.
Blood Coagul Fibrinolysis. 2005 Sep;16(6):447-52. doi: 10.1097/01.mbc.0000178827.52242.89.
Our aim was to establish whether differences in clotting times for recalcified blood and plasma samples might be explained by the use of different blood collection tubes. Samples obtained from different plastic vacuum tubes were recalcified and clotting times determined by free oscillation rheometry. The clotting times for blood collected in Vacutainer (Becton Dickinson, Rutherford, New Jersey, USA) or Vacuette (Greiner Bio-One, Kremsmünster, Austria) tubes decreased with time, with maximal effect after 30 min. Blood from Monovette (Sarstedt, Nümbrecht, Germany) tubes displayed longer clotting times, which did not decrease with time. Clotting times for plasma prepared after 1 h storage in Vacutainer or Vacuette tubes were unaffected by subsequent addition of corn trypsin inhibitor to inhibit factor XIIa, although an antibody against factor XI prolonged the clotting time markedly. In Monovette plasma, both corn trypsin inhibitor and anti-factor XI effectively prolonged the clotting time. When corn trypsin inhibitor or anti-factor XI was added to the tubes before blood collection, but both additions clearly prolonged the clotting times in all types of tubes, even though corn trypsin inhibitor was less effective in whole blood. Antibodies against human tissue factor did not affect the clotting times. The amounts of platelet or leukocyte microparticles in plasma were low and similar in all tubes. This indicates that blood collection in Vacutainer or Vacuette tubes induces a rapid activation of factor XII and factor XI.
我们的目的是确定重新钙化的血液和血浆样本的凝血时间差异是否可以通过使用不同的采血管来解释。从不同塑料真空管中获取的样本进行重新钙化，并通过自由振荡流变学测定凝血时间。用美国新泽西州卢瑟福的贝克顿·迪金森公司生产的Vacutainer管或奥地利克雷姆斯明斯特的格瑞纳生物-1公司生产的Vacuette管采集的血液，其凝血时间随时间缩短，30分钟后达到最大效果。用德国纽姆布雷希特的赛多利斯公司生产的Monovette管采集的血液显示出较长的凝血时间，且不随时间缩短。在Vacutainer管或Vacuette管中储存1小时后制备的血浆，随后添加玉米胰蛋白酶抑制剂以抑制因子XIIa，其凝血时间不受影响，尽管抗因子XI抗体显著延长了凝血时间。在Monovette血浆中，玉米胰蛋白酶抑制剂和抗因子XI均有效延长了凝血时间。在采血前将玉米胰蛋白酶抑制剂或抗因子XI添加到管中，两种添加物均明显延长了所有类型管中的凝血时间，尽管玉米胰蛋白酶抑制剂在全血中的效果较差。抗人组织因子抗体不影响凝血时间。所有管中血浆中的血小板或白细胞微粒数量均较低且相似。这表明用Vacutainer管或Vacuette管采血会诱导因子XII和因子XI的快速激活。
