Bajpai R, Waseem M, Gupta G S, Kaw J L
Industrial Toxicology Research Centre, Lucknow, India.
Toxicology. 1992;73(2):161-7. doi: 10.1016/0300-483x(92)90099-z.
Female wistar rats were inoculated intratracheally with 10 mg/ml suspensions of various dusts, viz: quartz, fly ash, mica and corundum in physiological saline. Biochemical markers of bronchoalveolar lavage fluid (BALF) were analysed 8 days after the instillation of the dusts. Elevated levels of proteins, sialic acid and phospholipid contents and the activity of lactate dehydrogenase correlated well with the degree of the known fibrogenic potential of different dusts in the lungs in the following order, quartz greater than fly ash greater than mica greater than corundum. beta-glucuronidase activity, was however, only elevated in the quartz inoculated group of rats. It is suggested that biochemical constituents of BALF analysed shortly after the exposure to different dusts can be useful to mirror alterations in the tissue response to mineral dusts.
将雌性Wistar大鼠经气管内接种10mg/ml的各种粉尘悬浮液，这些粉尘分别为：石英、粉煤灰、云母和刚玉的生理盐水悬浮液。在滴入粉尘8天后分析支气管肺泡灌洗液（BALF）的生化标志物。蛋白质、唾液酸和磷脂含量的升高以及乳酸脱氢酶的活性与不同粉尘在肺中已知的致纤维化潜能程度密切相关，顺序如下：石英＞粉煤灰＞云母＞刚玉。然而，β-葡萄糖醛酸酶活性仅在接种石英的大鼠组中升高。有人提出，在接触不同粉尘后不久分析BALF的生化成分有助于反映组织对矿物粉尘反应的变化。
