Tajima Mami, Amaya Misato, Sugita Takashi, Nishikawa Akemi, Tsuboi Ryoji
Department of Dermatology, Tokyo Medical University, Shinjuku-ku, Tokyo, Japan.
Nihon Ishinkin Gakkai Zasshi. 2005;46(3):193-6. doi: 10.3314/jjmm.46.193.
Malassezia spp. which normally colonize on the skin surface, are known as being either the cause or an exacerbating factor in a variety of skin conditions, including pityriasis versicolor, folliculitis, seborrheic dermatitis and atopic dermatitis. We report here three cases of Akatsuki disease (pomade crust). Scales and crusts were collected from the lesional skin and analyzed using a PCR-based non-culture method. Malassezia microflora in Akatsuki disease was compared to that of healthy subjects and atopic dermatitis patients. Samples were collected from upper and lower eyelids (Case 1), an operation scar (Case 2) and parietal scalp (Case 3). DNA was extracted from the scales and nested PCR was performed using specific primers for each species. Our analysis detected only M. obtusa and M. slooffiae in Cases 1 and 3 and only M. slooffiae in Case 2. Our previous data indicated that while M. globosa, M. restricta and M. sympodialis were common in healthy subjects, the two aforementioned species were rare, suggesting that the presence of M. obtusa and M. slooffiae in the subjects in the present study is correlated to the pathogenesis of Akatsuki disease.
马拉色菌属通常定植于皮肤表面，已知是多种皮肤疾病的病因或加重因素，包括花斑癣、毛囊炎、脂溢性皮炎和特应性皮炎。我们在此报告3例明月病（润发脂痂病）。从皮损处采集鳞屑和痂皮，采用基于聚合酶链反应（PCR）的非培养方法进行分析。将明月病患者的马拉色菌菌群与健康受试者和特应性皮炎患者的进行比较。样本取自上、下眼睑（病例1）、手术瘢痕（病例2）和头顶头皮（病例3）。从鳞屑中提取DNA，并使用针对每个菌种的特异性引物进行巢式PCR。我们的分析在病例1和病例3中仅检测到钝形马拉色菌和斯洛菲马拉色菌，在病例2中仅检测到斯洛菲马拉色菌。我们之前的数据表明，虽然球形马拉色菌、限制马拉色菌和合轴马拉色菌在健康受试者中很常见，但上述两种菌种很少见，这表明本研究中受试者体内钝形马拉色菌和斯洛菲马拉色菌的存在与明月病的发病机制相关。
