Patocchi A, Walser M, Tartarini S, Broggini G A L, Gennari F, Sansavini S, Gessler C
Plant Pathology, Institute of Plant Sciences, Swiss Federal Institute of Technology, Zuerich.
Genome. 2005 Aug;48(4):630-6. doi: 10.1139/g05-036.
For all known major apple scab resistance genes except Vr, molecular markers have been published. However, the precise position of some of these genes, in the apple genome, remains to be identified. Knowledge about the relative position of apple scab resistance genes is necessary to preliminarily evaluate the probability of success of their pyramidization. Pyramidization of different resistance genes into the same genotype is a reliable way to create cultivars with durable apple scab resistance. Applying the genome scanning approach (GSA), we identified the linkage group of the scab resistance gene Vm, derived from Malus micromalus, and we found a new molecular marker tightly associated with the gene. The simple sequence repeat Hi07h02, previously mapped on linkage group 17, cosegregates with the Vm gene (no recombinants in the 95 plants tested). The already published sequence-characterized amplified region Vm marker OPB12(687) was found to be linked at about 5 cM from the resistance gene and, therefore, this marker also maps on linkage group 17 of apple. This is the first report of the discovery of a major apple scab resistance gene on linkage group 17. The advantages of using GSA for the identification of molecular markers for qualitative traits are discussed.
除Vr外，所有已知的苹果黑星病主要抗性基因都已发表了分子标记。然而，其中一些基因在苹果基因组中的精确位置仍有待确定。了解苹果黑星病抗性基因的相对位置对于初步评估其聚合成功的可能性是必要的。将不同的抗性基因聚合到同一基因型中是培育具有持久苹果黑星病抗性品种的可靠方法。应用基因组扫描方法（GSA），我们鉴定了源自苹果属小金海棠的黑星病抗性基因Vm所在的连锁群，并发现了一个与该基因紧密连锁的新分子标记。先前定位在连锁群17上的简单序列重复Hi07h02与Vm基因共分离（在测试的95株植物中无重组体）。已发表的序列特征扩增区域Vm标记OPB12(687)被发现与抗性基因连锁，距离约为5厘摩，因此，该标记也定位在苹果的连锁群17上。这是在连锁群17上发现主要苹果黑星病抗性基因的首次报道。文中还讨论了使用GSA鉴定质量性状分子标记的优势。
