胰岛素样生长因子II信使核糖核酸在肝细胞癌患者肝癌组织及外周血中的表达与变化
Expression and alteration of insulin-like growth factor II-messenger RNA in hepatoma tissues and peripheral blood of patients with hepatocellular carcinoma.
作者信息
Dong Zhi-Zhen, Yao Deng-Fu, Yao Deng-Bing, Wu Xin-Hua, Wu Wei, Qiu Li-Wei, Jiang Dao-Rong, Zhu Jian-Hua, Meng Xian-Yong
机构信息
Department of Internal Medicine, Affiliated Hospital of Nantong University, Jiangsu Province, China.
出版信息
World J Gastroenterol. 2005 Aug 14;11(30):4655-60. doi: 10.3748/wjg.v11.i30.4655.
AIM
To investigate the clinical values of serum free insulin-like growth factor II (IGF-II) levels and IGF-II mRNA in hepatocellular carcinoma (HCC) tissues and peripheral blood for diagnosis of HCC and monitoring of extrahepatic metastasis.
METHODS
Total RNAs were extracted from HCC tissues or peripheral blood mononuclear cells from patients with HCC, liver diseases devoid of cancer, non-hepatic tumors, and healthy controls, respectively. IGF-II cDNAs were synthesized through random primers and reverse-transcriptase, amplified by polymerase chain reaction (PCR), and confirmed by DNA sequencing analysis. Serum free IGF-II levels in patients with different liver diseases were analyzed by an enzyme-linked immunosorbent assay.
RESULTS
The amplified fragments of IGF-II mRNA by RT-PCR were identical to originally designed ones with a size of 170 bp and confirmed by sequencing analysis. The dilution experiments revealed that the lowest sensitivity of our system was 2 ng/L of total RNA. The positive frequencies of IGF-II mRNA were 100% in HCC tissues, 53.3% in para-cancerous tissues, and 0% in non-cancerous tissues, respectively. The serum free IGF-II levels were significantly higher in HCC than those in chronic hepatitis or liver cirrhosis. The positive frequency of circulating IGF-II mRNA was 34.2% in HCC, no amplified fragment was found in other liver diseases, extrahepatic tumors, and normal controls, respectively. The circulating IGF-II mRNA correlated with the stage of HCC, and its positive rate was 100% in HCC with extrahepatic metastasis and 35.5% in HCC with AFP-negative. No significant correlation was found between tumor sizes and circulating IGF-II mRNA fragment.
CONCLUSION
The abnormal expressions of free IGF-II and IGF-II mRNA are useful tumor markers for HCC diagnosis, differentiation of extrahepatic metastasis and monitoring postoperative recurrence.
目的
探讨血清游离胰岛素样生长因子II（IGF-II）水平及IGF-II mRNA在肝细胞癌（HCC）组织和外周血中对HCC诊断及肝外转移监测的临床价值。
方法
分别从HCC患者、无癌肝病患者、非肝肿瘤患者及健康对照者的HCC组织或外周血单个核细胞中提取总RNA。通过随机引物和逆转录酶合成IGF-II cDNA，经聚合酶链反应（PCR）扩增，并经DNA测序分析确认。采用酶联免疫吸附测定法分析不同肝病患者血清游离IGF-II水平。
结果
RT-PCR扩增的IGF-II mRNA片段与原设计片段相同，大小为170 bp，经测序分析确认。稀释实验表明，本系统的最低灵敏度为2 ng/L总RNA。IGF-II mRNA在HCC组织中的阳性率为100%，癌旁组织中为53.3%，非癌组织中为0%。HCC患者血清游离IGF-II水平显著高于慢性肝炎或肝硬化患者。HCC中循环IGF-II mRNA的阳性率为34.2%，在其他肝病、肝外肿瘤及正常对照中均未发现扩增片段。循环IGF-II mRNA与HCC分期相关，在伴有肝外转移的HCC中阳性率为100%，在AFP阴性的HCC中为35.5%。肿瘤大小与循环IGF-II mRNA片段之间无显著相关性。
结论
游离IGF-II和IGF-II mRNA的异常表达是HCC诊断、肝外转移鉴别及术后复发监测的有用肿瘤标志物。
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