Zhang Gai-Ping, Li Qing-Mei, Yang Yan-Yan, Guo Jun-Qing, Li Xue-Wu, Deng Rui-Guang, Xiao Zhi-Jun, Xing Guang-Xu, Yang Ji-Fei, Zhao Dong, Cai Shu-Jun, Zang Wei-Min
Institute of Biotechnology, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China.
Avian Dis. 2005 Jun;49(2):177-81. doi: 10.1637/7272-090704R.
A rapid diagnostic strip for chicken infectious bursal disease (IBD) was developed based on membrane chromatography using high-affinity monoclonal antibodies directed to chicken infectious bursal disease virus (IBDV). The diagnostic strip has high specificity for detection of chicken IBDV antigen and recognizes a variety of the virus isolates, including virulent and attenuated strains, with no cross-reactivity to other viruses, such as Newcastle disease virus, Marek's disease virus, infectious bronchitis virus, infectious laryngotracheitis virus, and egg-drop-syndrome virus. The results showed that its specificity was highly consistent with the agar-gel precipitation test (AGP). The diagnostic strip detected as low as 800 median egg lethal dose (ELD50) viruses in the IBDV BC6/85-infected sample, which was comparable with AC-ELISA (400 ELD50) and 32 times more sensitive than the AGP test (2.56 x 10(4) ELD50). In experimental infection, IBDV was detected in the bursa as early as 36 hr postinfection with the diagnostic strip before the clinical signs and gross lesions appeared. It takes only 1-2 min to do a strip test to detect chicken IBDV antigen after the specimen is grounded in a whirl pack with finger massage.
基于膜色谱法，利用针对鸡传染性法氏囊病病毒（IBDV）的高亲和力单克隆抗体，研制出一种用于鸡传染性法氏囊病（IBD）的快速诊断试纸条。该诊断试纸条对鸡IBDV抗原检测具有高特异性，能识别多种病毒分离株，包括强毒株和弱毒株，与其他病毒如新城疫病毒、马立克氏病病毒、传染性支气管炎病毒、传染性喉气管炎病毒和产蛋下降综合征病毒无交叉反应。结果表明，其特异性与琼脂凝胶沉淀试验（AGP）高度一致。该诊断试纸条在IBDV BC6/85感染的样本中能检测到低至800个鸡胚半数致死量（ELD50）的病毒，与AC-ELISA（400 ELD50）相当，比AGP试验（2.56×10⁴ ELD50）敏感32倍。在实验感染中，早在感染后36小时，在临床症状和肉眼病变出现之前，用诊断试纸条就能在法氏囊中检测到IBDV。将标本在旋转包装中用手指按摩研磨后，只需1 - 2分钟即可通过试纸条检测鸡IBDV抗原。
