Kemona-Chetnik Izabela, Bodzenta-Lukaszyk Anna, Kucharewicz Iwona, Rogalewska Anna Maria
Klinika Alergologii i Chorób Wewnetrznych, Akademii Medycznej w Białymstoku.
Przegl Lek. 2005;62(2):98-101.
Allergic asthma is characterized by bronchial inflammation and repair processes at the same time, that cause increased airway obstruction. Recent evidences suggest that monocytes and macrophages may play important role in allergic inflammation. Activated by proinflammatory factors they can express tissue factor (TF) and tissue factor pathway inhibitor (TFPI). The aim of the present study was to evaluate TF and TFPI in plasma of bronchial asthma patients. The study was performed on 17 asthma patients with documented Dermatophagoides pteronyssinus (Dp) allergy. 15 healthy persons served as negative control. Specific bronchial challenge with Dp extracts was performed in asthma patients. Blood was collected before allergen challenge--A0, during the early asthmatic reaction (EAR)--A0 during the late asthmatic reaction (LAR)--A2, and 24 hours after administration of the first allergen dose--A3. The concentrations of TF and TFPI were measured by ELISA method. The concentration of TF in asthmatic patients was significantly higher as compared with healthy controls. In patients that developed only EAR, the concentration of TF increased at A1, then decreased at time when LAR should be developed (A2) and it was comparable with beginning values 24 hours after starting the challenge. In patients that developed EAR and LAR, the mean TF concentration increased during EAR (A,) with subsequent decline during LAR (A2). 24 hours after starting the challenge the TF concentration was higher than beginning values (A3). The concentration of TFPI in patients that developed only EAR was significantly higher than the values in healthy controls. It decreased during EAR (A1) and increased 24 hours after starting the challenge (A3). The mean concentration of TFPI in patients that developed EAR and LAR was not significantly higher than the values in healthy controls. It increased during EAR and LAR with the highest values 24 hours after starting the challenge. Coagulation system seems to be activated during allergic inflammation after allergen challenge. Increased levels of TF and TFPI in asthma patients may be connected with chronic bronchial inflammation and remodeling of bronchial wall.
过敏性哮喘的特征是支气管炎症和修复过程同时存在，这会导致气道阻塞加重。最近的证据表明，单核细胞和巨噬细胞可能在过敏性炎症中起重要作用。它们被促炎因子激活后可表达组织因子（TF）和组织因子途径抑制剂（TFPI）。本研究的目的是评估支气管哮喘患者血浆中的TF和TFPI。该研究对17例有明确的屋尘螨（Dp）过敏记录的哮喘患者进行。15名健康人作为阴性对照。对哮喘患者进行了Dp提取物的特异性支气管激发试验。在过敏原激发前采集血液——A0，在早期哮喘反应（EAR）期间——A1，在晚期哮喘反应（LAR）期间——A2，以及在首次给予过敏原剂量后24小时——A3。通过ELISA法测量TF和TFPI的浓度。哮喘患者的TF浓度与健康对照相比显著更高。在仅发生EAR的患者中，TF浓度在A1时升高，然后在应发生LAR的时间（A2）下降，并且在激发开始24小时后与初始值相当。在发生EAR和LAR的患者中，平均TF浓度在EAR（A1）期间升高，随后在LAR（A2）期间下降。激发开始24小时后TF浓度高于初始值（A3）。仅发生EAR的患者中TFPI浓度显著高于健康对照的值。它在EAR（A1）期间下降，并在激发开始24小时后（A3）升高。发生EAR和LAR的患者中TFPI的平均浓度与健康对照的值相比没有显著更高。它在EAR和LAR期间升高，在激发开始24小时后达到最高值。在过敏原激发后的过敏性炎症期间，凝血系统似乎被激活。哮喘患者TF和TFPI水平的升高可能与慢性支气管炎症和支气管壁重塑有关。
