Müller M, Lepom P
Institut für Futterproduktion Paulinenaue, Deutschland.
Zentralbl Mikrobiol. 1992;147(3-4):197-206.
Analytical methods are described for detection of the Alternaria mycotoxins alternariol (AOH), alternariol monomethyl ether (AME), altenuene (ALT) and tenuazonic acid (TeA) in natural and semisynthetic laboratory cultures. After extraction and purification of the crude extract by column chromatography on silica gel the qualitative and quantitative analyses were carried out by thin layer (TLC)- and high performance liquid chromatography (HPLC). HPLC separations were achieved using a Hypersil ODS column with methanol/water containing a complexing agent as eluent. Detection at 340 nm (AOH, AME, ALT) and 280 nm (TeA), respectively, has proved to be favourably. AME and TeA were produced in high purity and high yields as standard substances by two Alternaria strains. The identity of the toxins could be confirmed by EI-, CI- and FAB-mass spectrometry.
本文描述了用于检测天然和半合成实验室培养物中链格孢霉菌毒素——交链孢酚（AOH）、交链孢酚单甲醚（AME）、细偶氮酸（ALT）和细交链孢菌酮酸（TeA）的分析方法。通过硅胶柱色谱法对粗提物进行萃取和纯化后，采用薄层色谱（TLC）和高效液相色谱（HPLC）进行定性和定量分析。使用Hypersil ODS柱，以含有络合剂的甲醇/水作为洗脱剂进行HPLC分离。分别在340nm（AOH、AME、ALT）和280nm（TeA）处进行检测，结果证明效果良好。两种链格孢菌株以高纯度和高产量生产出了作为标准物质的AME和TeA。毒素的身份可通过电子轰击质谱（EI-MS）、化学电离质谱（CI-MS）和快原子轰击质谱（FAB-MS）来确认。
