Härtig W, Oklejewicz M, Strijkstra A M, Boerema A S, Stieler J, Arendt T
Department of Neurochemistry, Paul Flechsig Institute for Brain Research, University of Leipzig, Jahnallee 59, D-04109 Leipzig, Germany.
Brain Res. 2005 Sep 14;1056(1):100-4. doi: 10.1016/j.brainres.2005.07.017.
Paired helical filaments formed by the abnormally phosphorylated microtubule-associated tau are a main sign of Alzheimer's disease and other neurodegenerative disorders. The hippocampal CA3 region, a brain region with a high degree of synaptic plasticity, is known to be strongly involved in tau hyperphosphorylation in several neurodegenerative diseases. In addition, reversible tau phosphorylation was observed during hibernation in European ground squirrels. The present study provides data on the tau phosphorylation status in the hippocampus of euthermic Syrian hamsters (Mesocricetus auratus), laboratory animals potentially prone to hibernation. Mossy fibers in the CA3 region of all investigated hamsters were immunostained using an antiserum detecting phospho-serine 199 of tau. A similar staining pattern was obtained with CP-13 detecting phospho-serine 202. In contrast, the monoclonal antibody AT8, recognizing both phosphorylated serine 202 and threonine 205, stained the CA3 region only in old hamsters. These findings implicate an additional link between aging, tau phosphorylation and synaptic plasticity. Furthermore, the presented data allow analyses whether tau phosphorylation is reversible in these facultative hibernators and versatile laboratory animal as it was recently shown for the hibernation cycle of European ground squirrels.
由异常磷酸化的微管相关tau蛋白形成的双螺旋丝是阿尔茨海默病和其他神经退行性疾病的主要标志。海马CA3区是一个具有高度突触可塑性的脑区，已知在几种神经退行性疾病中与tau蛋白过度磷酸化密切相关。此外，在欧洲地松鼠冬眠期间观察到tau蛋白磷酸化是可逆的。本研究提供了关于正常体温的叙利亚仓鼠（金仓鼠）海马中tau蛋白磷酸化状态的数据，叙利亚仓鼠是可能易于冬眠的实验动物。使用检测tau蛋白丝氨酸199磷酸化的抗血清对所有研究仓鼠CA3区的苔藓纤维进行免疫染色。用检测丝氨酸202磷酸化的CP - 13获得了类似的染色模式。相比之下，识别磷酸化丝氨酸202和苏氨酸205的单克隆抗体AT8仅在老年仓鼠的CA3区染色。这些发现暗示了衰老、tau蛋白磷酸化和突触可塑性之间的另一种联系。此外，正如最近在欧洲地松鼠的冬眠周期中所显示的那样，所呈现的数据允许分析在这些兼性冬眠动物和通用实验动物中tau蛋白磷酸化是否可逆。
