胶束电动色谱法分离测定药物制剂中的克霉唑、对羟基苯甲酸甲酯和对羟基苯甲酸丙酯。
Separation and determination of clotrimazole, methylparaben and propylparaben in pharmaceutical preparation by micellar electrokinetic chromatography.
作者信息
Hamoudová Rafífa, Pospísilová Marie, Kavalírová Andrea, Solich Petr, Sícha Jan
机构信息
Charles University, Faculty of Pharmacy, Department of Analytical Chemistry, Heyrovského 1203, CZ-500 05 Hradec Králové, Czech Republic.
出版信息
J Pharm Biomed Anal. 2006 Jan 23;40(1):215-9. doi: 10.1016/j.jpba.2005.07.001. Epub 2005 Aug 10.
In this study, micellar electrokinetic chromatography (MEKC) method was developed for the determination of clotrimazole (CLO), methylparaben (MP) and propylparaben (PP) in a pharmaceutical preparation. Separation was carried out in a fused silica capillary (60 cm x 75 microm i.d.) at 25 kV with UV detection at 212 nm. Optimized background electrolyte (BGE) was 15 mM phosphate buffer (pH 7.2) containing 30 mM sodium dodecyl sulfate (SDS) as a surfactant. Rectilinear calibration ranges were 50-500 mg l(-1) for CLO, 10-100 mg l(-1) for MP and 2.5-25 mg l(-1) for PP. The total analysis time was < 12 min.
在本研究中，开发了胶束电动色谱（MEKC）法用于测定药物制剂中的克霉唑（CLO）、对羟基苯甲酸甲酯（MP）和对羟基苯甲酸丙酯（PP）。在熔融石英毛细管（60 cm×75 μm内径）中于25 kV下进行分离，并在212 nm处进行紫外检测。优化后的背景电解质（BGE）是含有30 mM十二烷基硫酸钠（SDS）作为表面活性剂的15 mM磷酸盐缓冲液（pH 7.2）。克霉唑的线性校准范围为50 - 500 mg l(-1)，对羟基苯甲酸甲酯为10 - 100 mg l(-1)，对羟基苯甲酸丙酯为2.5 - 25 mg l(-1)。总分析时间<12分钟。
Zotero 插件