Wang Chun, Zhang Yong, Seng Ho Soon, Ngo Low Lee
Division of Bioengineering, Faculty of Engineering, National University of Singapore.
Biosens Bioelectron. 2006 Feb 15;21(8):1638-43. doi: 10.1016/j.bios.2005.07.008. Epub 2005 Aug 10.
Micropatterning of proteins on silica substrate was achieved using a new method. Proteins were first immobilized onto silica nanoparticles which were then dispensed into arrayed microwells on silicon. Atomic force microscopy (AFM), fluorescence microscopy and Fourier transform infrared (FTIR) spectroscopy were used to characterize the samples. The results showed that, compared to a planar surface, curved surfaces of nanoparticles provide more space for attaching proteins and thus increases the intensity of fluorescence signal. Furthermore, after attaching to silica nanoparticles, bovine serum albumin (BSA) maintains its major structure and the cytokine IFN-gamma maintains its ability to bind to its antibody. Use of this method can be extended to micropatterning of other biomolecules, such as DNA and enzymes.
利用一种新方法在二氧化硅基质上实现了蛋白质的微图案化。首先将蛋白质固定在二氧化硅纳米颗粒上，然后将其分配到硅片上排列好的微孔中。使用原子力显微镜（AFM）、荧光显微镜和傅里叶变换红外（FTIR）光谱对样品进行表征。结果表明，与平面表面相比，纳米颗粒的弯曲表面为附着蛋白质提供了更多空间，从而增加了荧光信号强度。此外，牛血清白蛋白（BSA）附着到二氧化硅纳米颗粒后，其主要结构得以保留，细胞因子干扰素-γ（IFN-γ）保持了与抗体结合的能力。该方法的应用可扩展到其他生物分子如DNA和酶的微图案化。
