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Extracellular matrix moieties, cytokines, and enzymes: dynamic effects on immune cell behavior and inflammation.细胞外基质成分、细胞因子和酶：对免疫细胞行为和炎症的动态影响。
J Leukoc Biol. 2000 Feb;67(2):149-59. doi: 10.1002/jlb.67.2.149.
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Role of neurotrophins and neurotrophins receptors in the in vitro invasion and heparanase production of human prostate cancer cells.神经营养因子及其受体在人前列腺癌细胞体外侵袭和乙酰肝素酶产生中的作用
Clin Exp Metastasis. 1999 Jun;17(4):307-14. doi: 10.1023/a:1006652605568.
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Mammalian heparanase: gene cloning, expression and function in tumor progression and metastasis.哺乳动物乙酰肝素酶：基因克隆、表达及其在肿瘤进展和转移中的作用
Nat Med. 1999 Jul;5(7):793-802. doi: 10.1038/10518.
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Modulation of fibroblast growth factor-2 receptor binding, dimerization, signaling, and angiogenic activity by a synthetic heparin-mimicking polyanionic compound.一种合成的类肝素聚阴离子化合物对成纤维细胞生长因子-2受体结合、二聚化、信号传导及血管生成活性的调节作用
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Heparan sulfate degradation: relation to tumor invasive and metastatic properties of mouse B16 melanoma sublines.
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乙酰肝素酶mRNA在反义寡核苷酸转染的人食管癌EC9706细胞中的表达。

Expression of heparanase mRNA in anti-sense oligonucleotide-transfected human esophageal cancer EC9706 cells.

作者信息

Chen Kui-Sheng, Zhang Lan, Tang Lin, Zhang Yun-Han, Gao Dong-Ling, Yan Liang, Zhang Lei

机构信息

Department of Pathology, the First Affiliated Hospital, Zhengzhou University, Key Laboratory of Henan Tumor Pathology, Zhengzhou 450052, Henan Province, China.

出版信息

World J Gastroenterol. 2005 Aug 21;11(31):4916-7. doi: 10.3748/wjg.v11.i31.4916.

DOI:10.3748/wjg.v11.i31.4916
PMID:16097072
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4398750/
Abstract

AIM

To investigate the effects of anti-sense oligonucleotides (ASODNs) on mRNA expression of heparanase in human esophageal cancer EC9706 cells.

METHODS

One non-sense oligonucleotide (N-ODN) and five ASODNs against different heparanase mRNA sites were transfected into EC9706 cells, then the expression of heparanase mRNA in EC9706 cells was studied by in situ hybridization.

RESULTS

The expression of heparanase mRNA could be inhibited by ASODNs. There was no significant difference among five ASODNs (P>0.05), but there was a significant difference between ASODNs and N-ODN or non-transfected group (ASODN1: 2.25+/-0.25, ASODN2: 2.21+/-0.23, ASODN3: 2.23+/-0.23, ASODN4: 2.25+/-0.24 vs N-ODN: 3.47+/-2.80 or non- transfected group: 3.51+/-2.93 respectively, P<0.05).

CONCLUSION

The expression of heparanase mRNA in EC9706 cells can be inhibited by ASODNs in vivo, and heparanase ASODNs can inhibit metastasis of esophageal squamous cell carcinoma or other tumors by inhibiting the expression of heparanase.

摘要

目的

研究反义寡核苷酸（ASODNs）对人食管癌EC9706细胞中乙酰肝素酶mRNA表达的影响。

方法

将一条无义寡核苷酸（N-ODN）和五条针对不同乙酰肝素酶mRNA位点的ASODNs转染入EC9706细胞，然后通过原位杂交研究EC9706细胞中乙酰肝素酶mRNA的表达。

结果

ASODNs可抑制乙酰肝素酶mRNA的表达。五条ASODNs之间差异无统计学意义（P>0.05），但ASODNs与N-ODN或未转染组之间差异有统计学意义（ASODN1：2.25±0.25，ASODN2：2.21±0.23，ASODN3：2.23±0.23，ASODN4：2.25±0.24分别与N-ODN：3.47±2.80或未转染组：3.51±2.93相比，P<0.05）。

结论

ASODNs在体内可抑制EC9706细胞中乙酰肝素酶mRNA的表达，乙酰肝素酶ASODNs可通过抑制乙酰肝素酶的表达来抑制食管鳞状细胞癌或其他肿瘤的转移。