乙酰肝素酶mRNA在反义寡核苷酸转染的人食管癌EC9706细胞中的表达。
Expression of heparanase mRNA in anti-sense oligonucleotide-transfected human esophageal cancer EC9706 cells.
作者信息
Chen Kui-Sheng, Zhang Lan, Tang Lin, Zhang Yun-Han, Gao Dong-Ling, Yan Liang, Zhang Lei
机构信息
Department of Pathology, the First Affiliated Hospital, Zhengzhou University, Key Laboratory of Henan Tumor Pathology, Zhengzhou 450052, Henan Province, China.
出版信息
World J Gastroenterol. 2005 Aug 21;11(31):4916-7. doi: 10.3748/wjg.v11.i31.4916.
AIM
To investigate the effects of anti-sense oligonucleotides (ASODNs) on mRNA expression of heparanase in human esophageal cancer EC9706 cells.
METHODS
One non-sense oligonucleotide (N-ODN) and five ASODNs against different heparanase mRNA sites were transfected into EC9706 cells, then the expression of heparanase mRNA in EC9706 cells was studied by in situ hybridization.
RESULTS
The expression of heparanase mRNA could be inhibited by ASODNs. There was no significant difference among five ASODNs (P>0.05), but there was a significant difference between ASODNs and N-ODN or non-transfected group (ASODN1: 2.25+/-0.25, ASODN2: 2.21+/-0.23, ASODN3: 2.23+/-0.23, ASODN4: 2.25+/-0.24 vs N-ODN: 3.47+/-2.80 or non- transfected group: 3.51+/-2.93 respectively, P<0.05).
CONCLUSION
The expression of heparanase mRNA in EC9706 cells can be inhibited by ASODNs in vivo, and heparanase ASODNs can inhibit metastasis of esophageal squamous cell carcinoma or other tumors by inhibiting the expression of heparanase.
目的
研究反义寡核苷酸（ASODNs）对人食管癌EC9706细胞中乙酰肝素酶mRNA表达的影响。
方法
将一条无义寡核苷酸（N-ODN）和五条针对不同乙酰肝素酶mRNA位点的ASODNs转染入EC9706细胞，然后通过原位杂交研究EC9706细胞中乙酰肝素酶mRNA的表达。
结果
ASODNs可抑制乙酰肝素酶mRNA的表达。五条ASODNs之间差异无统计学意义（P>0.05），但ASODNs与N-ODN或未转染组之间差异有统计学意义（ASODN1：2.25±0.25，ASODN2：2.21±0.23，ASODN3：2.23±0.23，ASODN4：2.25±0.24分别与N-ODN：3.47±2.80或未转染组：3.51±2.93相比，P<0.05）。
结论
ASODNs在体内可抑制EC9706细胞中乙酰肝素酶mRNA的表达，乙酰肝素酶ASODNs可通过抑制乙酰肝素酶的表达来抑制食管鳞状细胞癌或其他肿瘤的转移。
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