Hino Mami, Oda Mutsumi, Yoshida Aya, Nakata Kazue, Kohchi Chie, Nishizawa Takashi, Inagawa Hiroyuki, Hori Hitoshi, Makino Kimiko, Terada Hiroshi, Soma Gen-Ichiro
Institute for Health Sciences, Tokushima Bunri University, Nishihama, Yamashiro-cho, Tokushima-shi, 770-8514, Japan.
In Vivo. 2005 Sep-Oct;19(5):821-30.
Mycobacterium tuberculosis infection affects one-third of the world's population and causes the death of three million people each year. To clarify details of M. tuberculosis survival strategies, it is important to establish a suitable in vitro model that mimics a chronic infection in alveolar macrophages by M. tuberculosis. For this reason, we established a new in vitro model using a rat alveolar macrophage cell line, NR8383.
Basic characteristics, including phagocytotic ability and production of nitrogen oxide and tumor necrosis factor in response to several stimuli, of NR8383 cells were compared with those of primary alveolar macrophages. The course after phagocytosis of live or killed M. bovis bacilli Calmette-Guerin (BCG) was examined over 21 days using NR8383 cells as the host.
The characteristics that have been examined to date were nearly the same for both primary alveolar macrophage and NR8383 cells. Live BCG phagocytosed by NR8383 cells had successfully begun to grow in the cells within 7 days, while killed BCG were almost completely destroyed by 21 days.
BCG-infected NR8383 cells are potentially a suitable in vitro model that mimics a chronic infection with M tuberculosis.
结核分枝杆菌感染影响着全球三分之一的人口，每年导致三百万人死亡。为了阐明结核分枝杆菌生存策略的细节，建立一个能够模拟结核分枝杆菌在肺泡巨噬细胞中慢性感染的合适体外模型非常重要。因此，我们利用大鼠肺泡巨噬细胞系NR8383建立了一个新的体外模型。
将NR8383细胞的基本特性，包括吞噬能力以及对多种刺激产生一氧化氮和肿瘤坏死因子的能力，与原代肺泡巨噬细胞的相应特性进行比较。以NR8383细胞作为宿主，观察其吞噬活的或灭活的卡介苗（BCG）后21天内的变化过程。
迄今为止所检测的原代肺泡巨噬细胞和NR8383细胞的特性几乎相同。NR8383细胞吞噬的活卡介苗在7天内已成功开始在细胞内生长，而灭活卡介苗在21天时几乎被完全破坏。
卡介苗感染的NR8383细胞可能是一种模拟结核分枝杆菌慢性感染的合适体外模型。
