Tissot J D, Clément F, Schifferli J A, Frei P C, Hochstrasser D F, Schneider P
Centre de Transfusion Sanguine CRS, Lausanne, Switzerland.
Am J Hematol. 1992 Jul;40(3):171-5. doi: 10.1002/ajh.2830400304.
High-resolution two-dimensional gel electrophoresis (2-DGE) was used to analyse plasma samples and partially purified cold agglutinins (CA) obtained from two selected patients. Both presented an acute hemolytic anemia with CA of high thermal amplitude, normal immunoglobulin levels, no detectable paraproteinemia, and no clinical evidence of a malignant B-cell disorder. The electrophoretograms of their plasma showed evident alternations of the "normal" protein profile, which were directly related to hemolysis (absence of the spots of haptoglobin and in one case of those of hemopexin), but no monoclonal gammopathy. The electrophoretograms of their purified CA revealed two clearly different spot patterns respectively corresponding to a monoclonal IgM and to polyclonal IgM. These results show that the clonality of CA associated with hemolytic anemia can be easily determined by 2-DGE. This technique may be very useful to discriminate chronic cold agglutinin disease in the early phase from "parainfectious" CA.
采用高分辨率二维凝胶电泳（2-DGE）分析了从两名选定患者获得的血浆样本和部分纯化的冷凝集素（CA）。两名患者均表现为急性溶血性贫血，伴有高热幅度的CA、正常免疫球蛋白水平、未检测到副蛋白血症，且无恶性B细胞疾病的临床证据。他们血浆的电泳图谱显示“正常”蛋白质谱有明显变化，这与溶血直接相关（缺乏触珠蛋白斑点，一例还缺乏血红素结合蛋白斑点），但无单克隆丙种球蛋白病。他们纯化的CA的电泳图谱显示出两种明显不同的斑点模式，分别对应单克隆IgM和多克隆IgM。这些结果表明，通过2-DGE可以轻松确定与溶血性贫血相关的CA的克隆性。该技术对于在早期区分慢性冷凝集素病与“副感染性”CA可能非常有用。
